A longitudinal study of seroreactivities to Plasmodium falciparum antigens in infants and children living in a holoendemic area of Liberia

TY - JOUR

T1 - A longitudinal study of seroreactivities to Plasmodium falciparum antigens in infants and children living in a holoendemic area of Liberia

AU - Hogh, B

AU - Marbiah, N T

AU - Petersen, E

AU - Perlmann, H

AU - Dolopaye, E

AU - Hanson, A P

AU - Bjorkman, A

AU - Perlmann, P

PY - 1991/2

Y1 - 1991/2

N2 - Investigators studied 348 children age 0-10 years, living in a holoendemic area of Liberia, for parasitological, serological and clinical parameters. The age-specific parasite rate increased towards the 7-10 year-old age group in which it was 86.8%. The geometrical mean parasite density decreased from the 3-4 year-old age group, in which fewer episodes of clinical malaria were observed. Antibodies to crude Plasmodium falciparum parasite antigens were detected in all children. The (EENV)6 seropositive rate was a maximum of 67.9% in the 3-11 month-old age group. It declined to a minimum of 31.7% in the 5-6 years age group after which it increased slowly in the 7-10 years age group. Antibodies to the synthetic peptide (NANP)6 showed a steady seropositive rate after the age of 3 months, between 30.0% and 39.3% in all the age groups up to 10 years. No statistically significant correlation was found between seropositivity to (EENV)6 and malarial parasitemia. In contrast, a statistically significant positive correlation was found between seropositivity to (NANP)6 and parasite rates. The antibody response for the individual child was transient to both Pf155/RESA, measured by immunofluorescence, and to (EENV)6 and (NANP)6, measured by ELISA, especially in the younger age groups of this study population. Parasitological and clinical immunity developed before a stable antibody response to these defined malaria antigens was established. These antibodies may still contribute to the immune protection against malaria, but they were not reliable parameters for protective immunity in the population we studied.

AB - Investigators studied 348 children age 0-10 years, living in a holoendemic area of Liberia, for parasitological, serological and clinical parameters. The age-specific parasite rate increased towards the 7-10 year-old age group in which it was 86.8%. The geometrical mean parasite density decreased from the 3-4 year-old age group, in which fewer episodes of clinical malaria were observed. Antibodies to crude Plasmodium falciparum parasite antigens were detected in all children. The (EENV)6 seropositive rate was a maximum of 67.9% in the 3-11 month-old age group. It declined to a minimum of 31.7% in the 5-6 years age group after which it increased slowly in the 7-10 years age group. Antibodies to the synthetic peptide (NANP)6 showed a steady seropositive rate after the age of 3 months, between 30.0% and 39.3% in all the age groups up to 10 years. No statistically significant correlation was found between seropositivity to (EENV)6 and malarial parasitemia. In contrast, a statistically significant positive correlation was found between seropositivity to (NANP)6 and parasite rates. The antibody response for the individual child was transient to both Pf155/RESA, measured by immunofluorescence, and to (EENV)6 and (NANP)6, measured by ELISA, especially in the younger age groups of this study population. Parasitological and clinical immunity developed before a stable antibody response to these defined malaria antigens was established. These antibodies may still contribute to the immune protection against malaria, but they were not reliable parameters for protective immunity in the population we studied.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Protozoan/blood

KW - Antigens, Protozoan/immunology

KW - Antigens, Surface/immunology

KW - Child

KW - Child, Preschool

KW - Enzyme-Linked Immunosorbent Assay

KW - Fluorescent Antibody Technique

KW - Humans

KW - Infant

KW - Liberia

KW - Longitudinal Studies

KW - Malaria/epidemiology

KW - Molecular Sequence Data

KW - Oligopeptides/immunology

KW - Plasmodium falciparum/immunology

KW - Protozoan Proteins/immunology

M3 - Journal article

C2 - 2012263

SN - 0002-9637

VL - 44

SP - 191

EP - 200

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

IS - 2

ER -