A likelihood ratio test for species membership based on DNA sequence data

TY - JOUR

T1 - A likelihood ratio test for species membership based on DNA sequence data

AU - Matz, Mikhail V.

AU - Nielsen, Rasmus

N1 - Keywords: DNA barcoding likelihood ratio tests assignment of individuals coalescent simulations

PY - 2005

Y1 - 2005

N2 - DNA barcoding as an approach for species identification is rapidly increasing in popularity. However, it remains unclear which statistical procedures should accompany the technique to provide a measure of uncertainty. Here we describe a likelihood ratio test which can be used to test if a sampled sequence is a member of an a priori specified species. We investigate the performance of the test using coalescence simulations, as well as using the real data from butterflies and frogs representing two kinds of challenge for DNA barcoding: extremely low and extremely high levels of sequence variability.

AB - DNA barcoding as an approach for species identification is rapidly increasing in popularity. However, it remains unclear which statistical procedures should accompany the technique to provide a measure of uncertainty. Here we describe a likelihood ratio test which can be used to test if a sampled sequence is a member of an a priori specified species. We investigate the performance of the test using coalescence simulations, as well as using the real data from butterflies and frogs representing two kinds of challenge for DNA barcoding: extremely low and extremely high levels of sequence variability.

U2 - 10.1098/rstb.2005.1728

DO - 10.1098/rstb.2005.1728

M3 - Journal article

C2 - 16214754

SN - 0962-8436

VL - 360

SP - 1969

EP - 1974

JO - Philosophical Transactions of the Royal Society B: Biological Sciences

JF - Philosophical Transactions of the Royal Society B: Biological Sciences

IS - 1462

ER -