Abstract
Human leukocyte antigen-G (HLA-G) is a non-classical HLA class Ib molecule shown to exhibit immunomodulatory function in a wide range of immune-based disorders. A number of functional HLA-G gene polymorphisms have been identified, including a 14-bp insertion/deletion polymorphism in exon 8 of the 3' untranslated region of the HLA-G gene, which has been associated with HLA-G mRNA stability. Moreover, studies show that homozygosity for the 14-bp insertion/deletion polymorphism is associated with lower HLA-G mRNA and protein levels and unique alternative splicing patterns. Here, we introduce a quick and reliable method to screen for the HLA-G 14-bp insertion/deletion polymorphism using an optimized real-time polymerase chain reaction protocol. The genotyping assay has been validated by comparison with conventional methods. As results can be obtained within a few hours, the assay will have a potential for clinical use.
| Original language | English |
|---|---|
| Journal | HLA |
| Volume | 79 |
| Issue number | 3 |
| Pages (from-to) | 186-9 |
| Number of pages | 4 |
| ISSN | 2059-2302 |
| DOIs | |
| Publication status | Published - Mar 2012 |
Keywords
- 3' Untranslated Regions
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Genotyping Techniques
- HLA-G Antigens
- Humans
- Molecular Sequence Data
- Mutagenesis, Insertional
- Polymorphism, Genetic
- Real-Time Polymerase Chain Reaction
- Reproducibility of Results
- Sequence Deletion
- Time Factors