A conserved Oct4/POUV-dependent network links adhesion and migration to progenitor maintenance

Alessandra Livigni; Hanna Peradziryi; Alexei A Sharov; Gloryn Chia; Fella Hammachi; Rosa Portero Migueles; Woranop Sukparangsi; Salvatore Pernagallo; Mark Bradley; Jennifer Nichols; Minoru S H Ko; Joshua M Brickman

doi:10.1016/j.cub.2013.09.048

A conserved Oct4/POUV-dependent network links adhesion and migration to progenitor maintenance

Alessandra Livigni, Hanna Peradziryi, Alexei A Sharov, Gloryn Chia, Fella Hammachi, Rosa Portero Migueles, Woranop Sukparangsi, Salvatore Pernagallo, Mark Bradley, Jennifer Nichols, Minoru S H Ko, Joshua M Brickman

Stem Cell and developmental Biology - early embryonic lineage specification Lab

29 Citations (Scopus)

Abstract

BACKGROUND: The class V POU domain transcription factor Oct4 (Pou5f1) is a pivotal regulator of embryonic stem cell (ESC) self-renewal and reprogramming of somatic cells to induced pluripotent stem (iPS) cells. Oct4 is also an important evolutionarily conserved regulator of progenitor cell differentiation during embryonic development.

RESULTS: Here we examine the function of Oct4 homologs in Xenopus embryos and compare this to the role of Oct4 in maintaining mammalian embryo-derived stem cells. Based on a combination of expression profiling of Oct4/POUV-depleted Xenopus embryos and in silico analysis of existing mammalian Oct4 target data sets, we defined a set of evolutionary-conserved Oct4/POUV targets. Most of these targets were regulators of cell adhesion. This is consistent with Oct4/POUV phenotypes observed in the adherens junctions in Xenopus ectoderm, mouse embryonic, and epiblast stem cells. A number of these targets could rescue both Oct4/POUV phenotypes in cellular adhesion and multipotent progenitor cell maintenance, whereas expression of cadherins on their own could only transiently support adhesion and block differentiation in both ESC and Xenopus embryos.

CONCLUSIONS: Currently, the list of Oct4 transcriptional targets contains thousands of genes. Using evolutionary conservation, we identified a core set of functionally relevant factors that linked the maintenance of adhesion to Oct4/POUV. We found that the regulation of adhesion by the Oct4/POUV network occurred at both transcriptional and posttranslational levels and was required for pluripotency.

Original language	English
Journal	Current biology : CB
Volume	23
Issue number	22
Pages (from-to)	2233-44
Number of pages	12
ISSN	0960-9822
DOIs	https://doi.org/10.1016/j.cub.2013.09.048
Publication status	Published - 18 Nov 2013

Keywords

Adherens Junctions
Animals
Cadherins
Cell Adhesion
Cell Differentiation
Cell Movement
Cells, Cultured
Ectoderm
Embryo, Nonmammalian
Embryonic Stem Cells
Gastrula
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Octamer Transcription Factor-3
Xenopus Proteins
Xenopus laevis

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10.1016/j.cub.2013.09.048

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@article{336061a1ab6347118bf8b32ecda34c60,

title = "A conserved Oct4/POUV-dependent network links adhesion and migration to progenitor maintenance",

abstract = "BACKGROUND: The class V POU domain transcription factor Oct4 (Pou5f1) is a pivotal regulator of embryonic stem cell (ESC) self-renewal and reprogramming of somatic cells to induced pluripotent stem (iPS) cells. Oct4 is also an important evolutionarily conserved regulator of progenitor cell differentiation during embryonic development.RESULTS: Here we examine the function of Oct4 homologs in Xenopus embryos and compare this to the role of Oct4 in maintaining mammalian embryo-derived stem cells. Based on a combination of expression profiling of Oct4/POUV-depleted Xenopus embryos and in silico analysis of existing mammalian Oct4 target data sets, we defined a set of evolutionary-conserved Oct4/POUV targets. Most of these targets were regulators of cell adhesion. This is consistent with Oct4/POUV phenotypes observed in the adherens junctions in Xenopus ectoderm, mouse embryonic, and epiblast stem cells. A number of these targets could rescue both Oct4/POUV phenotypes in cellular adhesion and multipotent progenitor cell maintenance, whereas expression of cadherins on their own could only transiently support adhesion and block differentiation in both ESC and Xenopus embryos.CONCLUSIONS: Currently, the list of Oct4 transcriptional targets contains thousands of genes. Using evolutionary conservation, we identified a core set of functionally relevant factors that linked the maintenance of adhesion to Oct4/POUV. We found that the regulation of adhesion by the Oct4/POUV network occurred at both transcriptional and posttranslational levels and was required for pluripotency.",

keywords = "Adherens Junctions, Animals, Cadherins, Cell Adhesion, Cell Differentiation, Cell Movement, Cells, Cultured, Ectoderm, Embryo, Nonmammalian, Embryonic Stem Cells, Gastrula, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Octamer Transcription Factor-3, Xenopus Proteins, Xenopus laevis",

author = "Alessandra Livigni and Hanna Peradziryi and Sharov, {Alexei A} and Gloryn Chia and Fella Hammachi and Migueles, {Rosa Portero} and Woranop Sukparangsi and Salvatore Pernagallo and Mark Bradley and Jennifer Nichols and Ko, {Minoru S H} and Brickman, {Joshua M}",

year = "2013",

month = nov,

day = "18",

doi = "10.1016/j.cub.2013.09.048",

language = "English",

volume = "23",

pages = "2233--44",

journal = "Current biology : CB",

issn = "0960-9822",

publisher = "Cell Press",

number = "22",

}

TY - JOUR

T1 - A conserved Oct4/POUV-dependent network links adhesion and migration to progenitor maintenance

AU - Livigni, Alessandra

AU - Peradziryi, Hanna

AU - Sharov, Alexei A

AU - Chia, Gloryn

AU - Hammachi, Fella

AU - Migueles, Rosa Portero

AU - Sukparangsi, Woranop

AU - Pernagallo, Salvatore

AU - Bradley, Mark

AU - Nichols, Jennifer

AU - Ko, Minoru S H

AU - Brickman, Joshua M

PY - 2013/11/18

Y1 - 2013/11/18

N2 - BACKGROUND: The class V POU domain transcription factor Oct4 (Pou5f1) is a pivotal regulator of embryonic stem cell (ESC) self-renewal and reprogramming of somatic cells to induced pluripotent stem (iPS) cells. Oct4 is also an important evolutionarily conserved regulator of progenitor cell differentiation during embryonic development.RESULTS: Here we examine the function of Oct4 homologs in Xenopus embryos and compare this to the role of Oct4 in maintaining mammalian embryo-derived stem cells. Based on a combination of expression profiling of Oct4/POUV-depleted Xenopus embryos and in silico analysis of existing mammalian Oct4 target data sets, we defined a set of evolutionary-conserved Oct4/POUV targets. Most of these targets were regulators of cell adhesion. This is consistent with Oct4/POUV phenotypes observed in the adherens junctions in Xenopus ectoderm, mouse embryonic, and epiblast stem cells. A number of these targets could rescue both Oct4/POUV phenotypes in cellular adhesion and multipotent progenitor cell maintenance, whereas expression of cadherins on their own could only transiently support adhesion and block differentiation in both ESC and Xenopus embryos.CONCLUSIONS: Currently, the list of Oct4 transcriptional targets contains thousands of genes. Using evolutionary conservation, we identified a core set of functionally relevant factors that linked the maintenance of adhesion to Oct4/POUV. We found that the regulation of adhesion by the Oct4/POUV network occurred at both transcriptional and posttranslational levels and was required for pluripotency.

AB - BACKGROUND: The class V POU domain transcription factor Oct4 (Pou5f1) is a pivotal regulator of embryonic stem cell (ESC) self-renewal and reprogramming of somatic cells to induced pluripotent stem (iPS) cells. Oct4 is also an important evolutionarily conserved regulator of progenitor cell differentiation during embryonic development.RESULTS: Here we examine the function of Oct4 homologs in Xenopus embryos and compare this to the role of Oct4 in maintaining mammalian embryo-derived stem cells. Based on a combination of expression profiling of Oct4/POUV-depleted Xenopus embryos and in silico analysis of existing mammalian Oct4 target data sets, we defined a set of evolutionary-conserved Oct4/POUV targets. Most of these targets were regulators of cell adhesion. This is consistent with Oct4/POUV phenotypes observed in the adherens junctions in Xenopus ectoderm, mouse embryonic, and epiblast stem cells. A number of these targets could rescue both Oct4/POUV phenotypes in cellular adhesion and multipotent progenitor cell maintenance, whereas expression of cadherins on their own could only transiently support adhesion and block differentiation in both ESC and Xenopus embryos.CONCLUSIONS: Currently, the list of Oct4 transcriptional targets contains thousands of genes. Using evolutionary conservation, we identified a core set of functionally relevant factors that linked the maintenance of adhesion to Oct4/POUV. We found that the regulation of adhesion by the Oct4/POUV network occurred at both transcriptional and posttranslational levels and was required for pluripotency.

KW - Adherens Junctions

KW - Animals

KW - Cadherins

KW - Cell Adhesion

KW - Cell Differentiation

KW - Cell Movement

KW - Cells, Cultured

KW - Ectoderm

KW - Embryo, Nonmammalian

KW - Embryonic Stem Cells

KW - Gastrula

KW - Gene Expression Regulation, Developmental

KW - Gene Regulatory Networks

KW - Octamer Transcription Factor-3

KW - Xenopus Proteins

KW - Xenopus laevis

U2 - 10.1016/j.cub.2013.09.048

DO - 10.1016/j.cub.2013.09.048

M3 - Journal article

C2 - 24210613

SN - 0960-9822

VL - 23

SP - 2233

EP - 2244

JO - Current biology : CB

JF - Current biology : CB

IS - 22

ER -

A conserved Oct4/POUV-dependent network links adhesion and migration to progenitor maintenance

Abstract

Keywords

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