A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa

Mikkel Winther Pedersen; Aurélien Ginolhac; Ludovic Antoine Alexandre Orlando; Jesper Olsen; Kenneth Andersen; Jakob Mørkøv Holm; Svend Visby Funder; Eske Willerslev; Kurt H. Kjær

doi:10.1016/j.quascirev.2013.06.006

A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa

Mikkel Winther Pedersen, Aurélien Ginolhac, Ludovic Antoine Alexandre Orlando, Jesper Olsen, Kenneth Andersen, Jakob Mørkøv Holm, Svend Visby Funder, Eske Willerslev, Kurt H. Kjær

58 Citations (Scopus)

Abstract

We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa.

Original language	English
Journal	Quaternary Science Reviews
Volume	75
Pages (from-to)	161-168
Number of pages	8
ISSN	0277-3791
DOIs	https://doi.org/10.1016/j.quascirev.2013.06.006
Publication status	Published - 1 Sept 2013

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Pedersen, M. W., Ginolhac, A., Orlando, L. A. A., Olsen, J., Andersen, K., Holm, J. M., Funder, S. V., Willerslev, E., & Kjær, K. H. (2013). A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa. Quaternary Science Reviews, 75, 161-168. https://doi.org/10.1016/j.quascirev.2013.06.006

A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa. / Pedersen, Mikkel Winther; Ginolhac, Aurélien; Orlando, Ludovic Antoine Alexandre et al.
In: Quaternary Science Reviews, Vol. 75, 01.09.2013, p. 161-168.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa.",

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AU - Andersen, Kenneth

AU - Holm, Jakob Mørkøv

AU - Funder, Svend Visby

AU - Willerslev, Eske

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A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa

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