A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

Joseph Bouskila; Pasha Javadi; Laurent Elkrief; Christian Casanova; Jean-François Bouchard; Maurice Ptito

doi:10.1155/2016/3127658

A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

Joseph Bouskila, Pasha Javadi, Laurent Elkrief, Christian Casanova, Jean-François Bouchard, Maurice Ptito^*

^*Corresponding author for this work

12 Citations (Scopus)

49 Downloads (Pure)

Abstract

The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys. We found that CB1R and FAAH distributions are well-preserved among these species. However, expression of NAPE-PLD is circumscribed to the photoreceptor layer only in monkeys. In contrast, CB2R expression is variable across these species; in mice, CB2R is found in retinal neurons but not in glial cells; in tree shrews, CB2R is expressed in Müller cell processes of the outer retina and in retinal neurons of the inner retina; in monkeys, CB2R is restricted to Müller cells. Finally, the expression patterns of MAGL and DAGLα are differently expressed across species. Overall, these results provide evidence that the eCB system is differently expressed in the retina of these mammals and suggest a distinctive role of eCBs in visual processing.

Original language	English
Article number	3127658
Journal	Neural Plasticity
Volume	2016
Number of pages	13
ISSN	2090-5904
DOIs	https://doi.org/10.1155/2016/3127658
Publication status	Published - 2016

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A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and MonkeysFinal published version, 11.8 MBLicence: CC BY

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title = "A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys",

abstract = "The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys. We found that CB1R and FAAH distributions are well-preserved among these species. However, expression of NAPE-PLD is circumscribed to the photoreceptor layer only in monkeys. In contrast, CB2R expression is variable across these species; in mice, CB2R is found in retinal neurons but not in glial cells; in tree shrews, CB2R is expressed in M{\"u}ller cell processes of the outer retina and in retinal neurons of the inner retina; in monkeys, CB2R is restricted to M{\"u}ller cells. Finally, the expression patterns of MAGL and DAGLα are differently expressed across species. Overall, these results provide evidence that the eCB system is differently expressed in the retina of these mammals and suggest a distinctive role of eCBs in visual processing.",

author = "Joseph Bouskila and Pasha Javadi and Laurent Elkrief and Christian Casanova and Jean-Fran{\c c}ois Bouchard and Maurice Ptito",

year = "2016",

doi = "10.1155/2016/3127658",

language = "English",

volume = "2016",

journal = "Neural Plasticity",

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T1 - A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

AU - Bouskila, Joseph

AU - Javadi, Pasha

AU - Elkrief, Laurent

AU - Casanova, Christian

AU - Bouchard, Jean-François

AU - Ptito, Maurice

PY - 2016

Y1 - 2016

N2 - The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys. We found that CB1R and FAAH distributions are well-preserved among these species. However, expression of NAPE-PLD is circumscribed to the photoreceptor layer only in monkeys. In contrast, CB2R expression is variable across these species; in mice, CB2R is found in retinal neurons but not in glial cells; in tree shrews, CB2R is expressed in Müller cell processes of the outer retina and in retinal neurons of the inner retina; in monkeys, CB2R is restricted to Müller cells. Finally, the expression patterns of MAGL and DAGLα are differently expressed across species. Overall, these results provide evidence that the eCB system is differently expressed in the retina of these mammals and suggest a distinctive role of eCBs in visual processing.

AB - The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys. We found that CB1R and FAAH distributions are well-preserved among these species. However, expression of NAPE-PLD is circumscribed to the photoreceptor layer only in monkeys. In contrast, CB2R expression is variable across these species; in mice, CB2R is found in retinal neurons but not in glial cells; in tree shrews, CB2R is expressed in Müller cell processes of the outer retina and in retinal neurons of the inner retina; in monkeys, CB2R is restricted to Müller cells. Finally, the expression patterns of MAGL and DAGLα are differently expressed across species. Overall, these results provide evidence that the eCB system is differently expressed in the retina of these mammals and suggest a distinctive role of eCBs in visual processing.

U2 - 10.1155/2016/3127658

DO - 10.1155/2016/3127658

M3 - Journal article

C2 - 26977322

AN - SCOPUS:84959199553

SN - 2090-5904

VL - 2016

JO - Neural Plasticity

JF - Neural Plasticity

M1 - 3127658

ER -

A Comparative Analysis of the Endocannabinoid System in the Retina of Mice, Tree Shrews, and Monkeys

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