A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula

Feng Feng; Jongho Sun; Guru, V Radhakrishnan; Tak Lee; Zoltan Bozsoki; Sebastien Fort; Aleksander Gavrin; Kira Gysel; Mikkel B. Thygesen; Kasper Rojkjaer Andersen; Simona Radutoiu; Jens Stougaard; Giles E. D. Oldroyd

doi:10.1038/s41467-019-12999-5

A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula

Feng Feng, Jongho Sun, Guru, V Radhakrishnan, Tak Lee, Zoltan Bozsoki, Sebastien Fort, Aleksander Gavrin, Kira Gysel, Mikkel B. Thygesen, Kasper Rojkjaer Andersen, Simona Radutoiu, Jens Stougaard, Giles E. D. Oldroyd

Department of Chemistry

28 Citations (Scopus)

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Abstract

Plants associate with beneficial arbuscular mycorrhizal fungi facilitating nutrient acquisition. Arbuscular mycorrhizal fungi produce chitooligosaccharides (COs) and lipo-chitooligosaccharides (LCOs), that promote symbiosis signalling with resultant oscillations in nuclear-associated calcium. The activation of symbiosis signalling must be balanced with activation of immunity signalling, which in fungal interactions is promoted by COs resulting from the chitinaceous fungal cell wall. Here we demonstrate that COs ranging from CO4-CO8 can induce symbiosis signalling in Medicago truncatula. CO perception is a function of the receptor-like kinases MtCERK1 and LYR4, that activate both immunity and symbiosis signalling. A combination of LCOs and COs act synergistically to enhance symbiosis signalling and suppress immunity signalling and receptors involved in both CO and LCO perception are necessary for mycorrhizal establishment. We conclude that LCOs, when present in a mix with COs, drive a symbiotic outcome and this mix of signals is essential for arbuscular mycorrhizal establishment.

Original language	English
Article number	5047
Journal	Nature Communications
Volume	10
Number of pages	12
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-019-12999-5
Publication status	Published - 1 Dec 2019

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Feng, F., Sun, J., Radhakrishnan, G. V., Lee, T., Bozsoki, Z., Fort, S., Gavrin, A., Gysel, K., Thygesen, M. B., Andersen, K. R., Radutoiu, S., Stougaard, J., & Oldroyd, G. E. D. (2019). A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula. Nature Communications, 10, [5047]. https://doi.org/10.1038/s41467-019-12999-5

Feng, F, Sun, J, Radhakrishnan, GV, Lee, T, Bozsoki, Z, Fort, S, Gavrin, A, Gysel, K, Thygesen, MB, Andersen, KR, Radutoiu, S, Stougaard, J & Oldroyd, GED 2019, 'A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula', Nature Communications, vol. 10, 5047. https://doi.org/10.1038/s41467-019-12999-5

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title = "A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula",

abstract = "Plants associate with beneficial arbuscular mycorrhizal fungi facilitating nutrient acquisition. Arbuscular mycorrhizal fungi produce chitooligosaccharides (COs) and lipo-chitooligosaccharides (LCOs), that promote symbiosis signalling with resultant oscillations in nuclear-associated calcium. The activation of symbiosis signalling must be balanced with activation of immunity signalling, which in fungal interactions is promoted by COs resulting from the chitinaceous fungal cell wall. Here we demonstrate that COs ranging from CO4-CO8 can induce symbiosis signalling in Medicago truncatula. CO perception is a function of the receptor-like kinases MtCERK1 and LYR4, that activate both immunity and symbiosis signalling. A combination of LCOs and COs act synergistically to enhance symbiosis signalling and suppress immunity signalling and receptors involved in both CO and LCO perception are necessary for mycorrhizal establishment. We conclude that LCOs, when present in a mix with COs, drive a symbiotic outcome and this mix of signals is essential for arbuscular mycorrhizal establishment.",

author = "Feng Feng and Jongho Sun and Radhakrishnan, {Guru, V} and Tak Lee and Zoltan Bozsoki and Sebastien Fort and Aleksander Gavrin and Kira Gysel and Thygesen, {Mikkel B.} and Andersen, {Kasper Rojkjaer} and Simona Radutoiu and Jens Stougaard and Oldroyd, {Giles E. D.}",

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T1 - A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula

AU - Feng, Feng

AU - Sun, Jongho

AU - Radhakrishnan, Guru, V

AU - Lee, Tak

AU - Bozsoki, Zoltan

AU - Fort, Sebastien

AU - Gavrin, Aleksander

AU - Gysel, Kira

AU - Thygesen, Mikkel B.

AU - Andersen, Kasper Rojkjaer

AU - Radutoiu, Simona

AU - Stougaard, Jens

AU - Oldroyd, Giles E. D.

PY - 2019/12/1

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N2 - Plants associate with beneficial arbuscular mycorrhizal fungi facilitating nutrient acquisition. Arbuscular mycorrhizal fungi produce chitooligosaccharides (COs) and lipo-chitooligosaccharides (LCOs), that promote symbiosis signalling with resultant oscillations in nuclear-associated calcium. The activation of symbiosis signalling must be balanced with activation of immunity signalling, which in fungal interactions is promoted by COs resulting from the chitinaceous fungal cell wall. Here we demonstrate that COs ranging from CO4-CO8 can induce symbiosis signalling in Medicago truncatula. CO perception is a function of the receptor-like kinases MtCERK1 and LYR4, that activate both immunity and symbiosis signalling. A combination of LCOs and COs act synergistically to enhance symbiosis signalling and suppress immunity signalling and receptors involved in both CO and LCO perception are necessary for mycorrhizal establishment. We conclude that LCOs, when present in a mix with COs, drive a symbiotic outcome and this mix of signals is essential for arbuscular mycorrhizal establishment.

AB - Plants associate with beneficial arbuscular mycorrhizal fungi facilitating nutrient acquisition. Arbuscular mycorrhizal fungi produce chitooligosaccharides (COs) and lipo-chitooligosaccharides (LCOs), that promote symbiosis signalling with resultant oscillations in nuclear-associated calcium. The activation of symbiosis signalling must be balanced with activation of immunity signalling, which in fungal interactions is promoted by COs resulting from the chitinaceous fungal cell wall. Here we demonstrate that COs ranging from CO4-CO8 can induce symbiosis signalling in Medicago truncatula. CO perception is a function of the receptor-like kinases MtCERK1 and LYR4, that activate both immunity and symbiosis signalling. A combination of LCOs and COs act synergistically to enhance symbiosis signalling and suppress immunity signalling and receptors involved in both CO and LCO perception are necessary for mycorrhizal establishment. We conclude that LCOs, when present in a mix with COs, drive a symbiotic outcome and this mix of signals is essential for arbuscular mycorrhizal establishment.

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DO - 10.1038/s41467-019-12999-5

M3 - Journal article

C2 - 31695035

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

M1 - 5047

ER -

A combination of chitooligosaccharide and lipochitooligosaccharide recognition promotes arbuscular mycorrhizal associations in Medicago truncatula

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