A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans

Naja Zenius Jespersen; Therese Juhlin Larsen; Lone Peijs; Søren Daugaard; Preben Homøe; Annika Loft; Jasper de Jong; Neha Mathur; Barbara Cannon; Jan Nedergaard; Bente Klarlund Pedersen; Kirsten Møller; Camilla Charlotte Schéele

doi:10.1016/j.cmet.2013.04.011

A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans

Naja Zenius Jespersen, Therese Juhlin Larsen, Lone Peijs, Søren Daugaard, Preben Homøe, Annika Loft, Jasper de Jong, Neha Mathur, Barbara Cannon, Jan Nedergaard, Bente Klarlund Pedersen, Kirsten Møller, Camilla Charlotte Schéele

Department of Clinical Medicine

362 Citations (Scopus)

Abstract

Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.

Original language	English
Journal	Cell Metabolism
Volume	17
Issue number	5
Pages (from-to)	798-805
Number of pages	8
ISSN	1550-4131
DOIs	https://doi.org/10.1016/j.cmet.2013.04.011
Publication status	Published - 7 May 2013

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Jespersen, N. Z., Larsen, T. J., Peijs, L., Daugaard, S., Homøe, P., Loft, A., de Jong, J., Mathur, N., Cannon, B., Nedergaard, J., Pedersen, B. K., Møller, K., & Schéele, C. C. (2013). A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans. Cell Metabolism, 17(5), 798-805. https://doi.org/10.1016/j.cmet.2013.04.011

Jespersen, NZ, Larsen, TJ, Peijs, L, Daugaard, S, Homøe, P, Loft, A, de Jong, J, Mathur, N, Cannon, B, Nedergaard, J, Pedersen, BK, Møller, K & Schéele, CC 2013, 'A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans', Cell Metabolism, vol. 17, no. 5, pp. 798-805. https://doi.org/10.1016/j.cmet.2013.04.011

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title = "A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans",

abstract = "Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.",

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T1 - A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans

AU - Jespersen, Naja Zenius

AU - Larsen, Therese Juhlin

AU - Peijs, Lone

AU - Daugaard, Søren

AU - Homøe, Preben

AU - Loft, Annika

AU - de Jong, Jasper

AU - Mathur, Neha

AU - Cannon, Barbara

AU - Nedergaard, Jan

AU - Pedersen, Bente Klarlund

AU - Møller, Kirsten

AU - Schéele, Camilla Charlotte

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N2 - Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.

AB - Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.

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EP - 805

JO - Cell Metabolism

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ER -

A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans

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