Abstract
In this paper, we describe a capillary-based microfluidic device utilizing flow induced dispersion analysis (FIDA) for quantitative characterization of biomarkers. The microfluidic device is fabricated by micromilling technology and has incorporated buried optical fibers for light detection. The angle and distance between the fiber guiding the excitation light source and the fiber collecting fluorescent emission light were optimized to enhance signal-to-noise ratio (SNR) and limit of detection (LOD). The prototype achieves a LOD of 50 nM for the fluorescein indicator by using a low-cost Miniature Fiber Optic Spectrometer. The FIDA-based procedure employing fluorescein as the indicator and human serum albumin (HSA) as the analyte is carried out in the microfluidic device.
| Original language | English |
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| Title of host publication | 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 |
| Number of pages | 4 |
| Publication date | 2 Sept 2013 |
| Pages | 1054-1057 |
| Article number | 6559903 |
| ISBN (Print) | 9781467363525 |
| DOIs | |
| Publication status | Published - 2 Sept 2013 |
| Event | 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China Duration: 7 Apr 2013 → 10 Apr 2013 |
Conference
| Conference | 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 |
|---|---|
| Country/Territory | China |
| City | Suzhou |
| Period | 07/04/2013 → 10/04/2013 |
| Sponsor | Institute of Electrical and Electronics Engineers (IEEE), IEEE Nanotechnology Council (NTC), Peking University, Soochow University, Chinese Academy of Sciences |
Keywords
- flow induced dispersion analysis
- microfluidics
- optical fibers