A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria

Rosanna Catherine Hennessy; Line Christiansen; Stefan Olsson; Peter Stougaard

doi:10.1016/j.mimet.2017.11.024

A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria

Rosanna Catherine Hennessy, Line Christiansen, Stefan Olsson, Peter Stougaard

Microbial Ecology and Biotechnology

2 Citations (Scopus)

Abstract

Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.

Original language	English
Journal	Journal of Microbiological Methods
Volume	144
Pages (from-to)	173-176
Number of pages	4
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2017.11.024
Publication status	Published - Jan 2018

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@article{01c0023512454a938c47aef79c8aa3e8,

title = "A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria",

abstract = "Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.",

author = "Hennessy, {Rosanna Catherine} and Line Christiansen and Stefan Olsson and Peter Stougaard",

year = "2018",

month = jan,

doi = "10.1016/j.mimet.2017.11.024",

language = "English",

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journal = "Journal of Microbiological Methods",

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T1 - A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria

AU - Hennessy, Rosanna Catherine

AU - Christiansen, Line

AU - Olsson, Stefan

AU - Stougaard, Peter

PY - 2018/1

Y1 - 2018/1

N2 - Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.

AB - Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.

U2 - 10.1016/j.mimet.2017.11.024

DO - 10.1016/j.mimet.2017.11.024

M3 - Journal article

C2 - 29203144

SN - 0167-7012

VL - 144

SP - 173

EP - 176

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria

Abstract

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