3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry

Ann Lin; Frøydis Sved Skottvoll; Simon Rayner; Stig Pedersen-Bjergaard; Gareth Sullivan; Stefan Krauss; Steven Ray Wilson; Sean Harrison

doi:10.1002/elps.201900170

3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry

Ann Lin, Frøydis Sved Skottvoll, Simon Rayner, Stig Pedersen-Bjergaard, Gareth Sullivan, Stefan Krauss, Steven Ray Wilson^*, Sean Harrison

^*Corresponding author for this work

12 Citations (Scopus)

Abstract

In vitro derived simplified 3D representations of human organs or organ functionalities are predicted to play a major role in disease modeling, drug development, and personalized medicine, as they complement traditional cell line approaches and animal models. The cells for 3D organ representations may be derived from primary tissues, embryonic stem cells or induced pluripotent stem cells and come in a variety of formats from aggregates of individual or mixed cell types, self-organizing in vitro developed “organoids” and tissue mimicking chips. Microfluidic devices that allow long-term maintenance and combination with other tissues, cells or organoids are commonly referred to as “microphysiological” or “organ-on-a-chip” systems. Organ-on-a-chip technology allows a broad range of “on-chip” and “off-chip” analytical techniques, whereby “on-chip” techniques offer the possibility of real time tracking and analysis. In the rapidly expanding tool kit for real time analytical assays, mass spectrometry, combined with “on-chip” electrophoresis, and other separation approaches offer attractive emerging tools. In this review, we provide an overview of current 3D cell culture models, a compendium of current analytical strategies, and we make a case for new approaches for integrating separation science and mass spectrometry in this rapidly expanding research field.

Original language	English
Journal	Electrophoresis
ISSN	0173-0835
DOIs	https://doi.org/10.1002/elps.201900170
Publication status	Published - 1 Jan 2020

Keywords

Chromatography
Electrophoresis
Mass spectrometry
Organ on a chip
Organoid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/elps.201900170

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abstract = "In vitro derived simplified 3D representations of human organs or organ functionalities are predicted to play a major role in disease modeling, drug development, and personalized medicine, as they complement traditional cell line approaches and animal models. The cells for 3D organ representations may be derived from primary tissues, embryonic stem cells or induced pluripotent stem cells and come in a variety of formats from aggregates of individual or mixed cell types, self-organizing in vitro developed “organoids” and tissue mimicking chips. Microfluidic devices that allow long-term maintenance and combination with other tissues, cells or organoids are commonly referred to as “microphysiological” or “organ-on-a-chip” systems. Organ-on-a-chip technology allows a broad range of “on-chip” and “off-chip” analytical techniques, whereby “on-chip” techniques offer the possibility of real time tracking and analysis. In the rapidly expanding tool kit for real time analytical assays, mass spectrometry, combined with “on-chip” electrophoresis, and other separation approaches offer attractive emerging tools. In this review, we provide an overview of current 3D cell culture models, a compendium of current analytical strategies, and we make a case for new approaches for integrating separation science and mass spectrometry in this rapidly expanding research field.",

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AU - Pedersen-Bjergaard, Stig

AU - Sullivan, Gareth

AU - Krauss, Stefan

AU - Ray Wilson, Steven

AU - Harrison, Sean

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AB - In vitro derived simplified 3D representations of human organs or organ functionalities are predicted to play a major role in disease modeling, drug development, and personalized medicine, as they complement traditional cell line approaches and animal models. The cells for 3D organ representations may be derived from primary tissues, embryonic stem cells or induced pluripotent stem cells and come in a variety of formats from aggregates of individual or mixed cell types, self-organizing in vitro developed “organoids” and tissue mimicking chips. Microfluidic devices that allow long-term maintenance and combination with other tissues, cells or organoids are commonly referred to as “microphysiological” or “organ-on-a-chip” systems. Organ-on-a-chip technology allows a broad range of “on-chip” and “off-chip” analytical techniques, whereby “on-chip” techniques offer the possibility of real time tracking and analysis. In the rapidly expanding tool kit for real time analytical assays, mass spectrometry, combined with “on-chip” electrophoresis, and other separation approaches offer attractive emerging tools. In this review, we provide an overview of current 3D cell culture models, a compendium of current analytical strategies, and we make a case for new approaches for integrating separation science and mass spectrometry in this rapidly expanding research field.

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3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry

Abstract

Keywords

UN SDGs

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