Additional file 7 of Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancer

Additional file 7. Figure S4b and c: CAFs lack osteogenic differentiation capacity in culture and in vivo. b) Representative micrographs of sections from the xenograft in vivo bone formation assay immunoperoxidase-stained with a human-specific vimentin antibody for identification of the implanted cells and additional cellular staining with hematoxylin and eosin (H&E). Bone formation is absent in implants with crude, low passage primary CAFs (left), but forms upon grafting of bMSCs (right, arrow). Bar = 50 μm. c) Representative micrographs of sections from in vivo bone formation assay stained for human-specific vimentin and hematoxylin (upper) and H&E (lower), showing absence of bone formation by myCAFs (left panel), otherwise readily formed by bMSCs (right panel, white arrows). Bar = 50 μm.