Additional file 3 of High incidence of imperforate vagina in ADGRA3-deficient mice

Additional file 3: Figure S3. Ovarian and uterine morphology, weight of adult organs, body weight, and estrus cycle of experimental groups. (a) HE-stained whole ovary sections from Adgra3+/+, Adgra3-/-, and Adgra3-/-CV mice. (b) HE-stained sections of the middle part of the right uterine horn in Adgra3+/+, Adgra3-/-, and Adgra3-/-CV mice. The uterine morphology is disturbed in Adgra3-/-CV, possibly due to the large fluid accumulation within the uterine horns. (c-f) Weight of (c) ovary, (d) uterine horn, (e) kidney, and (f) brain collected from 8- to 10-week-old Adgra3+/+ (n = 9), Adgra3-/- (n = 5), and Adgra3-/-CV (n = 3) mice. (g) Body weight of the age subgroups used in this study. P15: Adgra3+/+ (n = 5) and Adgra3-/- (n = 6); 8- to 10 weeks: Adgra3+/+ (n = 9), Adgra3-/- (n = 5), Adgra3-/-CV (n = 3); 1 year: Adgra3+/+ (n = 9) and Adgra3-/- (n = 9). Each dot represents an individual animal. (h) The average length of each estrus cycle of Adgra3+/+ (n = 6) and Adgra3-/- (n = 4) mice with an open vagina. Each dot represents one cycle counted as the days from one proestrus to the next. (i) The frequency of estrus, diestrus, proestrus, and metestrus observed in Adgra3+/+ (n = 6) and Adgra3-/- (n = 4) females with an open vagina are presented as the number of times each phase was recorded over 17 days. Data are presented as mean ±SEM. ****p < 0.0001.