Additional file 1 of Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancer

Additional file 1. Figure S1a and b: Identification of CD105low and CD105high CAFs in culture and in situ. a) FACS plots of CAF2 (left, passage 13) and CAF3 (right, passage 14) single-cell suspended and labeled by immunofluorescence with a CD105 antibody (x-axis) versus forward scatter (y-axis). Circles indicate CD105low (CAFlow) and CD105high (CAFhigh) cells. b) Bar plot of quantification of percentage of α-sm actin positive cells in smears from FACS sorted CAF1 and CAF2 into CAFlow (gray bar) and CAFhigh (black bar). Note the relatively few α-sm actin positive cells among the CAFlow population in both CAF1 and CAF2. Quantification in CAF3 was not done.