Weight loss after gastric bypass surgery in women is followed by a metabolically favorable decrease in 11beta-hydroxysteroid dehydrogenase 1 expression in subcutaneous adipose tissue

TY - JOUR

T1 - Weight loss after gastric bypass surgery in women is followed by a metabolically favorable decrease in 11beta-hydroxysteroid dehydrogenase 1 expression in subcutaneous adipose tissue

AU - Simonyte, Kotryna

AU - Olsson, Tommy

AU - Näslund, Ingmar

AU - Angelhed, Jan-Erik

AU - Lönn, Lars

AU - Mattsson, Cecilia

AU - Rask, Eva

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Background and Aims: The role of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) in the pathogenesis of obesity has been elucidated in humans and in various rodent models. Obesity is accompanied by disturbances in glucocorticoid metabolism, circulating adipokine levels, and fatty acid (FA) reesterification. This study was undertaken to evaluate glucocorticoid metabolismin sc fat before and after weight loss and to explore putative associations between 11β-HSD1 and leptin, adiponectin, and FA recycling. Subjects and Methods: Twenty-seven obese (mean body mass index 44.4 ± 4.4 kg/m2) women underwent gastric bypass surgery. Subcutaneous fat biopsies were collected before and 2 yr after surgery. The expression of 11β-HSD1, leptin, adiponectin, and phosphoenolpyruvate carboxykinase (PEPCK) mRNA was evaluated with real-time PCR. Serum leptin and adiponectin protein levels were estimated by ELISA. Results: Two years after gastric bypass surgery, significant reductions were observed in the mean body mass index (from 44.4 to 30.8 kg/m2) and mean waist circumference (from 121.9 to 90.6 cm). After weight loss, 11β-HSD1 mRNA expression decreased 4-fold (P < 0.001). Both leptin and adiponectin mRNA expression decreased, with concomitantly decreased circulating leptin and increased circulating adiponectin levels. PEPCK mRNA expression did not change. Conclusion: Weight loss after gastric bypass surgery was followed by metabolically favorable changes in insulin sensitivity, circulating leptin and adiponectin, and peripheral glucocorticoid metabolism. A significant reduction in 11β-HSD1 expression was observed in sc adipose tissue after weight loss. This suggests that up-regulation of 11β-HSD1 is a consequence, rather than a cause, of obesity.

AB - Background and Aims: The role of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) in the pathogenesis of obesity has been elucidated in humans and in various rodent models. Obesity is accompanied by disturbances in glucocorticoid metabolism, circulating adipokine levels, and fatty acid (FA) reesterification. This study was undertaken to evaluate glucocorticoid metabolismin sc fat before and after weight loss and to explore putative associations between 11β-HSD1 and leptin, adiponectin, and FA recycling. Subjects and Methods: Twenty-seven obese (mean body mass index 44.4 ± 4.4 kg/m2) women underwent gastric bypass surgery. Subcutaneous fat biopsies were collected before and 2 yr after surgery. The expression of 11β-HSD1, leptin, adiponectin, and phosphoenolpyruvate carboxykinase (PEPCK) mRNA was evaluated with real-time PCR. Serum leptin and adiponectin protein levels were estimated by ELISA. Results: Two years after gastric bypass surgery, significant reductions were observed in the mean body mass index (from 44.4 to 30.8 kg/m2) and mean waist circumference (from 121.9 to 90.6 cm). After weight loss, 11β-HSD1 mRNA expression decreased 4-fold (P < 0.001). Both leptin and adiponectin mRNA expression decreased, with concomitantly decreased circulating leptin and increased circulating adiponectin levels. PEPCK mRNA expression did not change. Conclusion: Weight loss after gastric bypass surgery was followed by metabolically favorable changes in insulin sensitivity, circulating leptin and adiponectin, and peripheral glucocorticoid metabolism. A significant reduction in 11β-HSD1 expression was observed in sc adipose tissue after weight loss. This suggests that up-regulation of 11β-HSD1 is a consequence, rather than a cause, of obesity.

U2 - 10.1210/jc.2009-2472

DO - 10.1210/jc.2009-2472

M3 - Journal article

SN - 0021-972X

VL - 95

SP - 3527

EP - 3531

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 7

ER -