Viral glycoproteomes: Technologies for characterization and outlook for vaccine design

TY - JOUR

T1 - Viral glycoproteomes

T2 - Technologies for characterization and outlook for vaccine design

AU - Bagdonaite, Ieva

AU - Vakhrushev, Sergey Y.

AU - Joshi, Hiren J.

AU - Wandall, Hans H.

PY - 2018/12

Y1 - 2018/12

N2 - It has long been known that surface proteins of most enveloped viruses are covered with glycans. It has furthermore been demonstrated that glycosylation is essential for propagation and immune evasion for many viruses. The recent development of high-resolution mass spectrometry techniques has enabled identification not only of the precise structures but also the positions of such post-translational modifications on viruses, revealing substantial differences in extent of glycosylation and glycan maturation for different classes of viruses. In-depth characterization of glycosylation and other post-translational modifications of viral envelope glycoproteins is essential for rational design of vaccines and antivirals. In this Review, we provide an overview of techniques used to address viral glycosylation and summarize information on glycosylation of enveloped viruses representing ongoing public health challenges. Furthermore, we discuss how knowledge on glycosylation can be translated to means to prevent and combat viral infections.

AB - It has long been known that surface proteins of most enveloped viruses are covered with glycans. It has furthermore been demonstrated that glycosylation is essential for propagation and immune evasion for many viruses. The recent development of high-resolution mass spectrometry techniques has enabled identification not only of the precise structures but also the positions of such post-translational modifications on viruses, revealing substantial differences in extent of glycosylation and glycan maturation for different classes of viruses. In-depth characterization of glycosylation and other post-translational modifications of viral envelope glycoproteins is essential for rational design of vaccines and antivirals. In this Review, we provide an overview of techniques used to address viral glycosylation and summarize information on glycosylation of enveloped viruses representing ongoing public health challenges. Furthermore, we discuss how knowledge on glycosylation can be translated to means to prevent and combat viral infections.

KW - Enveloped virus

KW - Glycoproteomics

KW - Mass spectrometry

KW - Site-specific glycosylation

KW - Virus glycosylation

U2 - 10.1002/1873-3468.13177

DO - 10.1002/1873-3468.13177

M3 - Review

C2 - 29961944

AN - SCOPUS:85050467789

SN - 0014-5793

VL - 592

SP - 3898

EP - 3920

JO - FEBS Letters

JF - FEBS Letters

IS - 23

ER -