TY - JOUR
T1 - Validation of image cytometry for sperm concentration measurement
T2 - Comparison with manual counting of 4010 human semen samples
AU - Egeberg Palme, Dorte L.
AU - Johannsen, Trine Holm
AU - Petersen, Jørgen Holm
AU - Skakkebæk, Niels E.
AU - Juul, Anders
AU - Jørgensen, Niels
AU - Almstrup, Kristian
PY - 2017
Y1 - 2017
N2 - Sperm concentration is an essential parameter in the diagnostic evaluation of men from infertile couples. It is usually determined by manual counting using a hemocytometer, and is therefore both laborious and subjective. We have earlier shown that a newly developed image cytometry (IC) method may be used to determine sperm concentration. Here we present a validation of the IC method by analysis of 4010 semen samples. There was high agreement between IC and manual counting at sperm concentrations above 3 mill/ml and in samples with concentrations above 12 mill/ml the two methods can be used interchangeable. However, we found substantial differences in samples below 3 mill/ml. We also assessed the accuracy of the two methods by repeated measurements of 248 samples, which revealed that IC measurements seemed more accurate. Moreover, based on ten samples counted by several operators the IC method had a lower coefficient of variation than the manual method (5% vs 10%), indicating a better precision of the IC method. In conclusion, measurement of sperm concentration by IC can be used at concentrations above 3 mill/ml and seems more accurate and precise than manual counting, making it an attractive option in the daily clinical practice.
AB - Sperm concentration is an essential parameter in the diagnostic evaluation of men from infertile couples. It is usually determined by manual counting using a hemocytometer, and is therefore both laborious and subjective. We have earlier shown that a newly developed image cytometry (IC) method may be used to determine sperm concentration. Here we present a validation of the IC method by analysis of 4010 semen samples. There was high agreement between IC and manual counting at sperm concentrations above 3 mill/ml and in samples with concentrations above 12 mill/ml the two methods can be used interchangeable. However, we found substantial differences in samples below 3 mill/ml. We also assessed the accuracy of the two methods by repeated measurements of 248 samples, which revealed that IC measurements seemed more accurate. Moreover, based on ten samples counted by several operators the IC method had a lower coefficient of variation than the manual method (5% vs 10%), indicating a better precision of the IC method. In conclusion, measurement of sperm concentration by IC can be used at concentrations above 3 mill/ml and seems more accurate and precise than manual counting, making it an attractive option in the daily clinical practice.
KW - Image cytometry
KW - Manual counting
KW - Reproducibility
KW - Sperm concentration
U2 - 10.1016/j.cca.2017.02.014
DO - 10.1016/j.cca.2017.02.014
M3 - Journal article
C2 - 28242391
AN - SCOPUS:85016712216
SN - 0009-8981
VL - 468
SP - 114
EP - 119
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -