Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum

TY - JOUR

T1 - Validation of an ELISA for detection of neutrophil gelatinase-associated lipocalin (NGAL) in equine serum

AU - Jacobsen, Stine

AU - Berg, Lise C.

AU - Tvermose, Emil

AU - Laurberg, Malene B.

AU - van Galen, Gaby

PY - 2018/12

Y1 - 2018/12

N2 - Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives: The aim of the study was to validate a commercially available porcine-specific ELISA to measure serum concentrations of equine NGAL. Methods: Intra- and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results: Intra- and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions: Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA.

AB - Background: Neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives: The aim of the study was to validate a commercially available porcine-specific ELISA to measure serum concentrations of equine NGAL. Methods: Intra- and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results: Intra- and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions: Equine serum NGAL concentrations could be quantified reliably using the porcine-specific ELISA.

KW - Assay validation

KW - enzyme-linked immunosorbent assay

KW - horse

KW - kidney injury

KW - lipocalin 2

KW - neutrophil gelatinase-associated lipocalin

U2 - 10.1111/vcp.12670

DO - 10.1111/vcp.12670

M3 - Journal article

C2 - 30403420

AN - SCOPUS:85056163569

SN - 0275-6382

VL - 47

SP - 603

EP - 607

JO - Veterinary Clinical Pathology

JF - Veterinary Clinical Pathology

IS - 4

ER -