UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner

Minjie Qian; Irina Kalbina; Eva Rosenqvist; Marcel A. K. Jansen; Yuanwen Teng; Åke Strid

doi:10.1039/c8pp00480c

UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner

Minjie Qian, Irina Kalbina, Eva Rosenqvist, Marcel A. K. Jansen, Yuanwen Teng, Åke Strid

Afgrødevidenskab

8 Citationer (Scopus)

Abstract

Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400nm) or ultraviolet-B (280-315 nm) radiation. The expression pattern of all twelve CsPAL, threeCsC4H, and three CsCHS genes was established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30 fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B- containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for more detailed studies of the fine regulation of spatial and temporal expression of key genes. This in turn, can facilitate the quantitative investigation of the relationships between different promotor motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.

Originalsprog	Engelsk
Tidsskrift	Photochemical & Photobiological Sciences
Vol/bind	18
Udgave nummer	2
Sider (fra-til)	424-433
Antal sider	10
ISSN	1474-905X
DOI	https://doi.org/10.1039/c8pp00480c
Status	Udgivet - 2019

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10.1039/c8pp00480c

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title = "UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner",

abstract = "Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, the expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400 nm) or ultraviolet-B (280-315 nm) radiation. The expression patterns of all twelve CsPAL, three CsC4H, and three CsCHS genes were established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another pattern (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, and CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30-fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B-containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for combining more detailed studies of the morphology, physiological parameters and fine regulation of spatial and temporal expression of key genes. This, in turn, can facilitate the quantitative investigation of the relationships among different promoter motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.",

keywords = "Natural Sciences, Biological Sciences, Biochemistry And Molecular Biology, Naturvetenskap, Biologiska Vetenskaper, Biokemi Och Molekyl{\"a}rbiologi, Botany, Botanik, Biochemistry, Biokemi",

author = "Minjie Qian and Irina Kalbina and Eva Rosenqvist and Jansen, {Marcel A. K.} and Yuanwen Teng and {\AA}ke Strid",

year = "2019",

doi = "10.1039/c8pp00480c",

language = "English",

volume = "18",

pages = "424--433",

journal = "Photochemical & Photobiological Sciences",

issn = "1474-905X",

publisher = "Royal Society of Chemistry",

number = "2",

}

TY - JOUR

T1 - UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner

AU - Qian, Minjie

AU - Kalbina, Irina

AU - Rosenqvist, Eva

AU - Jansen, Marcel A. K.

AU - Teng, Yuanwen

AU - Strid, Åke

PY - 2019

Y1 - 2019

N2 - Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, the expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400 nm) or ultraviolet-B (280-315 nm) radiation. The expression patterns of all twelve CsPAL, three CsC4H, and three CsCHS genes were established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another pattern (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, and CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30-fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B-containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for combining more detailed studies of the morphology, physiological parameters and fine regulation of spatial and temporal expression of key genes. This, in turn, can facilitate the quantitative investigation of the relationships among different promoter motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.

AB - Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, the expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400 nm) or ultraviolet-B (280-315 nm) radiation. The expression patterns of all twelve CsPAL, three CsC4H, and three CsCHS genes were established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another pattern (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, and CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30-fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B-containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for combining more detailed studies of the morphology, physiological parameters and fine regulation of spatial and temporal expression of key genes. This, in turn, can facilitate the quantitative investigation of the relationships among different promoter motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.

KW - Natural Sciences

KW - Biological Sciences

KW - Biochemistry And Molecular Biology

KW - Naturvetenskap

KW - Biologiska Vetenskaper

KW - Biokemi Och Molekylärbiologi

KW - Botany

KW - Botanik

KW - Biochemistry

KW - Biokemi

U2 - 10.1039/c8pp00480c

DO - 10.1039/c8pp00480c

M3 - Journal article

SN - 1474-905X

VL - 18

SP - 424

EP - 433

JO - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

IS - 2

ER -

UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner

Abstract

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