Utility of X-chromosome SNPs in relationship testing

Carmen Tomas, Juan Jose Sanchez, J.A. Castro, C. Børsting, Niels Morling

3 Citationer (Scopus)

Abstract

X-chromosome markers may complement the results obtained from other genetic markers in complex relationship cases. Until now, reports on relationship testing using X-chromosome markers have mainly included data of short tandem repeats (STRs) while little data on single nucleotide polymorphisms (SNPs) in relationship testing have been published. We selected 25 highly polymorphic biallelic SNPs distributed through the human X-chromosome. One 25-plex PCR reaction and one 25-plex single base extension (SNaPshot) reaction were developed. The maximum size of the PCR products was 120ábp and the size of the SBE primers varied between 18 and 85 nucleotides. We analyzed the allele and haplotype frequencies in 1078 unrelated males. All the SNPs were polymorphic and the lowest minor allele frequency was 0.103. All the haplotypes were unique. The forensic parameters were calculated in the Danish and Somali populations. In the Danish population (Ná=á93), the power of discrimination (PD) in females was one in 4.4áÎá109 individuals and the PD in males was one in 2.6áÎá106. The PD in Somalis (Ná=á91) was one in 2.7áÎá109 in females and one in 1.7áÎá106 in males. Finally, we present an example of how the 25 X-chromosome SNP-multiplex may help to solve a complex immigration case
OriginalsprogEngelsk
TitelProgress in Forensic Genetics 12 : Proceedings of the 22nd International ISFG Congress
RedaktørerNiels Morling
Vol/bind1
ForlagElsevier
Publikationsdato2008
Sider528-530
StatusUdgivet - 2008
Begivenhed22nd International ISFG Congress - Copenhagen, Danmark
Varighed: 21 aug. 200725 aug. 2007

Konference

Konference22nd International ISFG Congress
Land/OmrådeDanmark
ByCopenhagen
Periode21/08/200725/08/2007
NavnForensic Science International: Genetics Supplement Series
Nummer1
Vol/bind1
ISSN1875-1768

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