TY - JOUR
T1 - Unliganded and substrate bound structures of the cellooligosaccharide active lytic polysaccharide monooxygenase LsAA9A at low pH
AU - Frandsen, Kristian Erik Høpfner
AU - Poulsen, Jens-Christian Navarro
AU - Tandrup, Tobias
AU - Lo Leggio, Leila
PY - 2017
Y1 - 2017
N2 - Lytic polysaccharide monooxygenases (LPMOs) have been found to be key components in microbial (bacterial and fungal) degradation of biomass. They are copper metalloenzymes that degrade polysaccharides oxidatively and act in synergy with glycoside hydrolases. Recently crystallographic studies carried out at pH 5.5 of the LPMO from Lentinus similis belonging to the fungal LPMO family AA9 have provided the first atomic resolution view of substrate-LPMO interactions. The LsAA9A structure presented here determined at pH 3.5 shows significant disorder of the active site in the absence of substrate ligand. Furthermore some differences are also observed in regards to substrate (cellohexaose) binding, although the major interaction with the N-terminal histidine remains unchanged.
AB - Lytic polysaccharide monooxygenases (LPMOs) have been found to be key components in microbial (bacterial and fungal) degradation of biomass. They are copper metalloenzymes that degrade polysaccharides oxidatively and act in synergy with glycoside hydrolases. Recently crystallographic studies carried out at pH 5.5 of the LPMO from Lentinus similis belonging to the fungal LPMO family AA9 have provided the first atomic resolution view of substrate-LPMO interactions. The LsAA9A structure presented here determined at pH 3.5 shows significant disorder of the active site in the absence of substrate ligand. Furthermore some differences are also observed in regards to substrate (cellohexaose) binding, although the major interaction with the N-terminal histidine remains unchanged.
KW - Cellohexaose interactions
KW - Lytic polysaccharide monooxygenases
KW - PH-dependent disorder
U2 - 10.1016/j.carres.2017.03.010
DO - 10.1016/j.carres.2017.03.010
M3 - Journal article
C2 - 28364950
AN - SCOPUS:85016489386
SN - 0008-6215
VL - 448
SP - 187
EP - 190
JO - Carbohydrate Research
JF - Carbohydrate Research
ER -