Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue

Claus Yding Andersen; M. Rosendahl; A.G. Byskov; A. Loft; C. Ottosen; M. Dueholm; Kirsten Louise Tryde Schmidt; Anders Nyboe Andersen; E. Ernst

Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue

Claus Yding Andersen, M. Rosendahl, A.G. Byskov, A. Loft, C. Ottosen, M. Dueholm, Kirsten Louise Tryde Schmidt, Anders Nyboe Andersen, E. Ernst

Institut for Klinisk Medicin

375 Citationer (Scopus)

Abstract

BACKGROUND: Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease. The present study reports ovarian activity and pregnancies following autotransplantation of frozen/thawed ovarian tissue. METHODS: One complete ovary was cryopreserved from each of six patients who were 26-35 years old prior to treatment. Tissue from three of the patients was transported 4-5 h on ice prior to cryopreservation. After a period of 17-32 months, orthotopic autotransplantation was performed replacing 20-60% of the tissue. Two patients received additional heterotopic transplants. RESULTS: In all cases, the tissue restored menstrual cyclicity 14-20 weeks following transplantation. Four of the six women conceived following assisted reproduction: two women (who had the tissue transported 4-5 h prior to cryopreservation) each, based on the orthotopic transplanted tissue, delivered one healthy child (February 2007 and January 2008); one woman miscarriaged in gestational Week 7; and the other had a positive hCG test but no clinical pregnancy. The remaining two women did not become pregnant. CONCLUSIONS: Two additional healthy children have been born as a result of the ovarian cryopreservation procedure. In both cases, the ovarian tissue was transported 4-5 h prior to freezing demonstrating that hospitals may offer cryopreservation without having the necessary expertise locally
Udgivelsesdato: 2008/10

Originalsprog	Engelsk
Tidsskrift	Human Reproduction
Vol/bind	23
Udgave nummer	10
Sider (fra-til)	2266-2272
Antal sider	6
ISSN	0268-1161
Status	Udgivet - 2008

Citationsformater

@article{737c1bf005b011deb05e000ea68e967b,

title = "Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue",

abstract = "BACKGROUND: Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease. The present study reports ovarian activity and pregnancies following autotransplantation of frozen/thawed ovarian tissue. METHODS: One complete ovary was cryopreserved from each of six patients who were 26-35 years old prior to treatment. Tissue from three of the patients was transported 4-5 h on ice prior to cryopreservation. After a period of 17-32 months, orthotopic autotransplantation was performed replacing 20-60% of the tissue. Two patients received additional heterotopic transplants. RESULTS: In all cases, the tissue restored menstrual cyclicity 14-20 weeks following transplantation. Four of the six women conceived following assisted reproduction: two women (who had the tissue transported 4-5 h prior to cryopreservation) each, based on the orthotopic transplanted tissue, delivered one healthy child (February 2007 and January 2008); one woman miscarriaged in gestational Week 7; and the other had a positive hCG test but no clinical pregnancy. The remaining two women did not become pregnant. CONCLUSIONS: Two additional healthy children have been born as a result of the ovarian cryopreservation procedure. In both cases, the ovarian tissue was transported 4-5 h prior to freezing demonstrating that hospitals may offer cryopreservation without having the necessary expertise locally Udgivelsesdato: 2008/10",

author = "Andersen, {Claus Yding} and M. Rosendahl and A.G. Byskov and A. Loft and C. Ottosen and M. Dueholm and Schmidt, {Kirsten Louise Tryde} and Andersen, {Anders Nyboe} and E. Ernst",

year = "2008",

language = "English",

volume = "23",

pages = "2266--2272",

journal = "Human Reproduction",

issn = "0268-1161",

publisher = "Oxford Academic",

number = "10",

}

TY - JOUR

T1 - Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue

AU - Andersen, Claus Yding

AU - Rosendahl, M.

AU - Byskov, A.G.

AU - Loft, A.

AU - Ottosen, C.

AU - Dueholm, M.

AU - Schmidt, Kirsten Louise Tryde

AU - Andersen, Anders Nyboe

AU - Ernst, E.

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease. The present study reports ovarian activity and pregnancies following autotransplantation of frozen/thawed ovarian tissue. METHODS: One complete ovary was cryopreserved from each of six patients who were 26-35 years old prior to treatment. Tissue from three of the patients was transported 4-5 h on ice prior to cryopreservation. After a period of 17-32 months, orthotopic autotransplantation was performed replacing 20-60% of the tissue. Two patients received additional heterotopic transplants. RESULTS: In all cases, the tissue restored menstrual cyclicity 14-20 weeks following transplantation. Four of the six women conceived following assisted reproduction: two women (who had the tissue transported 4-5 h prior to cryopreservation) each, based on the orthotopic transplanted tissue, delivered one healthy child (February 2007 and January 2008); one woman miscarriaged in gestational Week 7; and the other had a positive hCG test but no clinical pregnancy. The remaining two women did not become pregnant. CONCLUSIONS: Two additional healthy children have been born as a result of the ovarian cryopreservation procedure. In both cases, the ovarian tissue was transported 4-5 h prior to freezing demonstrating that hospitals may offer cryopreservation without having the necessary expertise locally Udgivelsesdato: 2008/10

AB - BACKGROUND: Cryopreservation of the ovarian cortex with subsequent autotransplantation has, on an experimental basis, been performed to preserve fertility in women being treated for a malignant disease. The present study reports ovarian activity and pregnancies following autotransplantation of frozen/thawed ovarian tissue. METHODS: One complete ovary was cryopreserved from each of six patients who were 26-35 years old prior to treatment. Tissue from three of the patients was transported 4-5 h on ice prior to cryopreservation. After a period of 17-32 months, orthotopic autotransplantation was performed replacing 20-60% of the tissue. Two patients received additional heterotopic transplants. RESULTS: In all cases, the tissue restored menstrual cyclicity 14-20 weeks following transplantation. Four of the six women conceived following assisted reproduction: two women (who had the tissue transported 4-5 h prior to cryopreservation) each, based on the orthotopic transplanted tissue, delivered one healthy child (February 2007 and January 2008); one woman miscarriaged in gestational Week 7; and the other had a positive hCG test but no clinical pregnancy. The remaining two women did not become pregnant. CONCLUSIONS: Two additional healthy children have been born as a result of the ovarian cryopreservation procedure. In both cases, the ovarian tissue was transported 4-5 h prior to freezing demonstrating that hospitals may offer cryopreservation without having the necessary expertise locally Udgivelsesdato: 2008/10

M3 - Journal article

SN - 0268-1161

VL - 23

SP - 2266

EP - 2272

JO - Human Reproduction

JF - Human Reproduction

IS - 10

ER -