Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors

Sara Bonde; Trine Berthing; Morten Hannibal Madsen; Tor Kristian Andersen; Nina Buch-Månson; Lei Guo; Xiaomei Li; F. Badique; K. Anselme; Jesper Nygård; Karen Laurence Martinez

doi:10.1021/am402070k

Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors

Sara Bonde, Trine Berthing, Morten Hannibal Madsen, Tor Kristian Andersen, Nina Buch-Månson, Lei Guo, Xiaomei Li, F. Badique, K. Anselme, Jesper Nygård, Karen Laurence Martinez

53 Citationer (Scopus)

Abstract

Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.

Originalsprog	Engelsk
Tidsskrift	A C S Applied Materials and Interfaces
Vol/bind	5
Udgave nummer	21
Sider (fra-til)	10510-10519
ISSN	1944-8244
DOI	https://doi.org/10.1021/am402070k
Status	Udgivet - 13 nov. 2013

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10.1021/am402070k

Citationsformater

Bonde, S., Berthing, T., Madsen, M. H., Andersen, T. K., Buch-Månson, N., Guo, L., Li, X., Badique, F., Anselme, K., Nygård, J., & Martinez, K. L. (2013). Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors. A C S Applied Materials and Interfaces, 5(21), 10510-10519. https://doi.org/10.1021/am402070k

Bonde, S, Berthing, T, Madsen, MH, Andersen, TK, Buch-Månson, N, Guo, L, Li, X, Badique, F, Anselme, K, Nygård, J & Martinez, KL 2013, 'Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors', A C S Applied Materials and Interfaces, bind 5, nr. 21, s. 10510-10519. https://doi.org/10.1021/am402070k

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title = "Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors",

abstract = "Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.",

author = "Sara Bonde and Trine Berthing and Madsen, {Morten Hannibal} and Andersen, {Tor Kristian} and Nina Buch-M{\aa}nson and Lei Guo and Xiaomei Li and F. Badique and K. Anselme and Jesper Nyg{\aa}rd and Martinez, {Karen Laurence}",

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AU - Bonde, Sara

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AU - Madsen, Morten Hannibal

AU - Andersen, Tor Kristian

AU - Buch-Månson, Nina

AU - Guo, Lei

AU - Li, Xiaomei

AU - Badique, F.

AU - Anselme, K.

AU - Nygård, Jesper

AU - Martinez, Karen Laurence

PY - 2013/11/13

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N2 - Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.

AB - Arrays of nanowires (NWs) are currently being established as vehicles for molecule delivery and electrical- and fluorescence-based platforms in the development of biosensors. It is conceivable that NW-based biosensors can be optimized through increased understanding of how the nanotopography influences the interfaced biological material. Using state-of-the-art homogenous NW arrays allow for a systematic investigation of how the broad range of NW densities used by the community influences cells. Here it is demonstrated that indium arsenide NW arrays provide a cell-promoting surface, which affects both cell division and focal adhesion up-regulation. Furthermore, a systematic variation in NW spacing affects both the detailed cell morphology and adhesion properties, where the latter can be predicted based on changes in free-energy states using the proposed theoretical model. As the NW density influences cellular parameters, such as cell size and adhesion tightness, it will be important to take NW density into consideration in the continued development of NW-based platforms for cellular applications, such as molecule delivery and electrical measurements.

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Tuning InAs Nanowire Density for HEK293 Cell Viability, Adhesion, and Morphology: Perspectives for Nanowire-Based Biosensors

Abstract

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