TY - JOUR
T1 - Triterpene Acids from Rose Hip Powder Inhibit Self-antigen- and LPS-induced Cytokine Production and CD4(+) T-cell Proliferation in Human Mononuclear Cell Cultures
AU - Saaby, Lasse
AU - Nielsen, Claus Henrik
N1 - Copyright © 2011 John Wiley & Sons, Ltd.
PY - 2012/8
Y1 - 2012/8
N2 - triterpene acid mixture consisting of oleanolic, ursolic and betulinic acid isolated from standardized rose hip powder (Ros canin L.) has been shown to inhibit interleukin (IL)-6 release from Mono Mac 6 cells. The present study examined the effects of the triterpene acid mixture on the cytokine production and proliferation of CD4+ T cells and CD19+ B cells induced by self-antigen, human thyroglobulin and by lipopolysaccharide in cultures of normal mononuclear cells. The triterpene acid mixture inhibited the production of tumor necrosis factor-α and IL-6 with estimated IC 50 values in the range 35-56 μg/mL, the Th1 cytokines interferon-γ and IL-2 (IC50 values 10-20 μg/mL) and the antiinflammatory cytokine IL-10 (IC50 values 18-21 μg/mL). Moreover, the mixture also inhibited CD4+ T-cell and CD19+ B-cell proliferation (IC50 value 22 and 12 μg/mL, respectively). Together, these dat demonstrate that oleanolic, ursolic and betulinic acid are active immunomodulatory constituents of the standardized rose hip powder. However, since the estimated IC50 values are in the μg/mL range, it is questionable whether the content of the triterpene acids in the standardized rose hip powder, alone, can explain the reported clinical effects.
AB - triterpene acid mixture consisting of oleanolic, ursolic and betulinic acid isolated from standardized rose hip powder (Ros canin L.) has been shown to inhibit interleukin (IL)-6 release from Mono Mac 6 cells. The present study examined the effects of the triterpene acid mixture on the cytokine production and proliferation of CD4+ T cells and CD19+ B cells induced by self-antigen, human thyroglobulin and by lipopolysaccharide in cultures of normal mononuclear cells. The triterpene acid mixture inhibited the production of tumor necrosis factor-α and IL-6 with estimated IC 50 values in the range 35-56 μg/mL, the Th1 cytokines interferon-γ and IL-2 (IC50 values 10-20 μg/mL) and the antiinflammatory cytokine IL-10 (IC50 values 18-21 μg/mL). Moreover, the mixture also inhibited CD4+ T-cell and CD19+ B-cell proliferation (IC50 value 22 and 12 μg/mL, respectively). Together, these dat demonstrate that oleanolic, ursolic and betulinic acid are active immunomodulatory constituents of the standardized rose hip powder. However, since the estimated IC50 values are in the μg/mL range, it is questionable whether the content of the triterpene acids in the standardized rose hip powder, alone, can explain the reported clinical effects.
U2 - 10.1002/ptr.3713
DO - 10.1002/ptr.3713
M3 - Journal article
C2 - 22170858
SN - 0951-418X
VL - 26
SP - 1142
EP - 1147
JO - Phytotherapy Research
JF - Phytotherapy Research
IS - 8
ER -