Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae

Yousof Tahtah; Kenneth Thermann Kongstad; Sileshi Gizachew Wubshet; Nils Nyberg; Louise H Jønsson; Anna K Jäger; Sun Qinglei; Dan Stærk

doi:10.1016/j.chroma.2015.07.010

Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae

Yousof Tahtah, Kenneth Thermann Kongstad, Sileshi Gizachew Wubshet, Nils Nyberg, Louise H Jønsson, Anna K Jäger, Sun Qinglei, Dan Stærk

34 Citationer (Scopus)

Abstract

In this work, development of a new microplate-based high-resolution profiling assay using recombinant human aldose reductase is presented. Used together with high-resolution radical scavenging and high-resolution α-glucosidase assays, it provided the first report of a triple aldose reductase/α-glucosidase/radical scavenging high-resolution inhibition profile - allowing proof of concept with Radix Scutellariae crude extract as a polypharmacological herbal drug. The triple bioactivity high-resolution profiles were used to pinpoint bioactive compounds, and subsequent structure elucidation was performed with hyphenated high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy. The only α-glucosidase inhibitor was baicalein, whereas main aldose reductase inhibitors in the crude extract were baicalein and skullcapflavone II, and main radical scavengers were ganhuangemin, viscidulin III, baicalin, oroxylin A 7-O-glucuronide, wogonoside, baicalein, wogonin, and skullcapflavone II.

Originalsprog	Engelsk
Tidsskrift	Journal of Chromatography A
Vol/bind	1408
Sider (fra-til)	125-132
Antal sider	8
ISSN	0021-9673
DOI	https://doi.org/10.1016/j.chroma.2015.07.010
Status	Udgivet - 2015

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10.1016/j.chroma.2015.07.010

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Citationsformater

Tahtah, Y., Kongstad, K. T., Wubshet, S. G., Nyberg, N., Jønsson, L. H., Jäger, A. K., Qinglei, S., & Stærk, D. (2015). Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae. Journal of Chromatography A, 1408, 125-132. https://doi.org/10.1016/j.chroma.2015.07.010

Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae. / Tahtah, Yousof; Kongstad, Kenneth Thermann; Wubshet, Sileshi Gizachew et al.
I: Journal of Chromatography A, Bind 1408, 2015, s. 125-132.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Tahtah, Y, Kongstad, KT, Wubshet, SG, Nyberg, N, Jønsson, LH, Jäger, AK, Qinglei, S & Stærk, D 2015, 'Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae', Journal of Chromatography A, bind 1408, s. 125-132. https://doi.org/10.1016/j.chroma.2015.07.010

Tahtah Y, Kongstad KT, Wubshet SG, Nyberg N, Jønsson LH, Jäger AK et al. Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae. Journal of Chromatography A. 2015;1408:125-132. doi: 10.1016/j.chroma.2015.07.010

Tahtah, Yousof ; Kongstad, Kenneth Thermann ; Wubshet, Sileshi Gizachew et al. / Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae. I: Journal of Chromatography A. 2015 ; Bind 1408. s. 125-132.

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title = "Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae",

abstract = "In this work, development of a new microplate-based high-resolution profiling assay using recombinant human aldose reductase is presented. Used together with high-resolution radical scavenging and high-resolution α-glucosidase assays, it provided the first report of a triple aldose reductase/α-glucosidase/radical scavenging high-resolution inhibition profile - allowing proof of concept with Radix Scutellariae crude extract as a polypharmacological herbal drug. The triple bioactivity high-resolution profiles were used to pinpoint bioactive compounds, and subsequent structure elucidation was performed with hyphenated high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy. The only α-glucosidase inhibitor was baicalein, whereas main aldose reductase inhibitors in the crude extract were baicalein and skullcapflavone II, and main radical scavengers were ganhuangemin, viscidulin III, baicalin, oroxylin A 7-O-glucuronide, wogonoside, baicalein, wogonin, and skullcapflavone II. ",

author = "Yousof Tahtah and Kongstad, {Kenneth Thermann} and Wubshet, {Sileshi Gizachew} and Nils Nyberg and J{\o}nsson, {Louise H} and J{\"a}ger, {Anna K} and Sun Qinglei and Dan St{\ae}rk",

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doi = "10.1016/j.chroma.2015.07.010",

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AU - Tahtah, Yousof

AU - Kongstad, Kenneth Thermann

AU - Wubshet, Sileshi Gizachew

AU - Nyberg, Nils

AU - Jønsson, Louise H

AU - Jäger, Anna K

AU - Qinglei, Sun

AU - Stærk, Dan

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N2 - In this work, development of a new microplate-based high-resolution profiling assay using recombinant human aldose reductase is presented. Used together with high-resolution radical scavenging and high-resolution α-glucosidase assays, it provided the first report of a triple aldose reductase/α-glucosidase/radical scavenging high-resolution inhibition profile - allowing proof of concept with Radix Scutellariae crude extract as a polypharmacological herbal drug. The triple bioactivity high-resolution profiles were used to pinpoint bioactive compounds, and subsequent structure elucidation was performed with hyphenated high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy. The only α-glucosidase inhibitor was baicalein, whereas main aldose reductase inhibitors in the crude extract were baicalein and skullcapflavone II, and main radical scavengers were ganhuangemin, viscidulin III, baicalin, oroxylin A 7-O-glucuronide, wogonoside, baicalein, wogonin, and skullcapflavone II.

AB - In this work, development of a new microplate-based high-resolution profiling assay using recombinant human aldose reductase is presented. Used together with high-resolution radical scavenging and high-resolution α-glucosidase assays, it provided the first report of a triple aldose reductase/α-glucosidase/radical scavenging high-resolution inhibition profile - allowing proof of concept with Radix Scutellariae crude extract as a polypharmacological herbal drug. The triple bioactivity high-resolution profiles were used to pinpoint bioactive compounds, and subsequent structure elucidation was performed with hyphenated high-performance liquid chromatography – high-resolution mass spectrometry – solid-phase extraction – nuclear magnetic resonance spectroscopy. The only α-glucosidase inhibitor was baicalein, whereas main aldose reductase inhibitors in the crude extract were baicalein and skullcapflavone II, and main radical scavengers were ganhuangemin, viscidulin III, baicalin, oroxylin A 7-O-glucuronide, wogonoside, baicalein, wogonin, and skullcapflavone II.

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DO - 10.1016/j.chroma.2015.07.010

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SN - 0021-9673

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EP - 132

JO - Journal of Chromatography A

JF - Journal of Chromatography A

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