Transplantation of amniotic membrane to the subretinal space in pigs

Jens Folke Kiilgaard; Erik Scherfig; Jan Ulrik Prause; Morten la Cour

doi:10.1155/2012/716968

Transplantation of amniotic membrane to the subretinal space in pigs

Jens Folke Kiilgaard, Erik Scherfig, Jan Ulrik Prause, Morten la Cour

Institut for Neurovidenskab

18 Citationer (Scopus)

Abstract

Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch's membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P < 0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.

Originalsprog	Engelsk
Tidsskrift	Stem Cells International
Vol/bind	2012
Udgave nummer	716968
Sider (fra-til)	1-5
Antal sider	5
ISSN	1687-966X
DOI	https://doi.org/10.1155/2012/716968
Status	Udgivet - 2012

Adgang til dokumentet

10.1155/2012/716968

Citationsformater

@article{445c7eef92d24aadbab2691a7b84d2ae,

title = "Transplantation of amniotic membrane to the subretinal space in pigs",

abstract = "Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch's membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P < 0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.",

author = "Kiilgaard, {Jens Folke} and Erik Scherfig and Prause, {Jan Ulrik} and {la Cour}, Morten",

year = "2012",

doi = "10.1155/2012/716968",

language = "English",

volume = "2012",

pages = "1--5",

journal = "Stem Cells International",

issn = "1687-966X",

publisher = "Hindawi Publishing Corporation",

number = "716968",

}

TY - JOUR

T1 - Transplantation of amniotic membrane to the subretinal space in pigs

AU - Kiilgaard, Jens Folke

AU - Scherfig, Erik

AU - Prause, Jan Ulrik

AU - la Cour, Morten

PY - 2012

Y1 - 2012

N2 - Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch's membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P < 0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.

AB - Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing. Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch's membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space. Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P < 0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE. Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.

U2 - 10.1155/2012/716968

DO - 10.1155/2012/716968

M3 - Journal article

C2 - 22550516

SN - 1687-966X

VL - 2012

SP - 1

EP - 5

JO - Stem Cells International

JF - Stem Cells International

IS - 716968

ER -

Transplantation of amniotic membrane to the subretinal space in pigs

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater