Translational coupling of nasST expression in Azotobacter vinelandii prevents overexpression of the nasT gene

Baomin Wang; Christopher Günther T Rensing; Leland S. Pierson; Hui Zhao; Christina  Kennedy

doi:10.1111/1574-6968.12621

Translational coupling of nasST expression in Azotobacter vinelandii prevents overexpression of the nasT gene

Baomin Wang, Christopher Günther T Rensing, Leland S. Pierson , Hui Zhao, Christina Kennedy

Microbial Ecology and Biotechnology

1 Citationer (Scopus)

Abstract

The nasST operon encodes the transcriptional regulators of assimilatory nitrate reductase operons in phylogenetically diverse bacteria. NasT is a RNA-binding antiterminator and helps RNA polymerase read through the regulatory terminator sequences upstream of the structural genes. NasS senses nitrate and nitrite and regulates the activity of NasT through stoichiometric interaction. In this study, we analyzed the nasST sequence in Azotobacter vinelandii and revealed that the nasS and nasT genes overlap by 19 nucleotides. Our genetic analyses suggested that translational initiation of NasT was coupled with NasS translation, a regulatory mechanism that prevents overproduction of NasT. The significance of tight control of nasT expression was demonstrated in a nasT-overexpression strain, where expression of the assimilatory nitrate reductase operon was deregulated.

Originalsprog	Engelsk
Tidsskrift	F E M S Microbiology Letters
Vol/bind	361
Udgave nummer	2
Sider (fra-til)	123-130
Antal sider	8
ISSN	0378-1097
DOI	https://doi.org/10.1111/1574-6968.12621
Status	Udgivet - 1 dec. 2014

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10.1111/1574-6968.12621

https://academic.oup.com/femsle/article-pdf/361/2/123/19126644/361-2-123.pdf

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abstract = "The nasST operon encodes the transcriptional regulators of assimilatory nitrate reductase operons in phylogenetically diverse bacteria. NasT is a RNA-binding antiterminator and helps RNA polymerase read through the regulatory terminator sequences upstream of the structural genes. NasS senses nitrate and nitrite and regulates the activity of NasT through stoichiometric interaction. In this study, we analyzed the nasST sequence in Azotobacter vinelandii and revealed that the nasS and nasT genes overlap by 19 nucleotides. Our genetic analyses suggested that translational initiation of NasT was coupled with NasS translation, a regulatory mechanism that prevents overproduction of NasT. The significance of tight control of nasT expression was demonstrated in a nasT-overexpression strain, where expression of the assimilatory nitrate reductase operon was deregulated.",

author = "Baomin Wang and Rensing, {Christopher G{\"u}nther T} and Pierson, {Leland S.} and Hui Zhao and Christina Kennedy",

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T1 - Translational coupling of nasST expression in Azotobacter vinelandii prevents overexpression of the nasT gene

AU - Wang, Baomin

AU - Rensing, Christopher Günther T

AU - Pierson , Leland S.

AU - Zhao, Hui

AU - Kennedy, Christina

PY - 2014/12/1

Y1 - 2014/12/1

N2 - The nasST operon encodes the transcriptional regulators of assimilatory nitrate reductase operons in phylogenetically diverse bacteria. NasT is a RNA-binding antiterminator and helps RNA polymerase read through the regulatory terminator sequences upstream of the structural genes. NasS senses nitrate and nitrite and regulates the activity of NasT through stoichiometric interaction. In this study, we analyzed the nasST sequence in Azotobacter vinelandii and revealed that the nasS and nasT genes overlap by 19 nucleotides. Our genetic analyses suggested that translational initiation of NasT was coupled with NasS translation, a regulatory mechanism that prevents overproduction of NasT. The significance of tight control of nasT expression was demonstrated in a nasT-overexpression strain, where expression of the assimilatory nitrate reductase operon was deregulated.

AB - The nasST operon encodes the transcriptional regulators of assimilatory nitrate reductase operons in phylogenetically diverse bacteria. NasT is a RNA-binding antiterminator and helps RNA polymerase read through the regulatory terminator sequences upstream of the structural genes. NasS senses nitrate and nitrite and regulates the activity of NasT through stoichiometric interaction. In this study, we analyzed the nasST sequence in Azotobacter vinelandii and revealed that the nasS and nasT genes overlap by 19 nucleotides. Our genetic analyses suggested that translational initiation of NasT was coupled with NasS translation, a regulatory mechanism that prevents overproduction of NasT. The significance of tight control of nasT expression was demonstrated in a nasT-overexpression strain, where expression of the assimilatory nitrate reductase operon was deregulated.

U2 - 10.1111/1574-6968.12621

DO - 10.1111/1574-6968.12621

M3 - Journal article

C2 - 25302751

SN - 0378-1097

VL - 361

SP - 123

EP - 130

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

IS - 2

ER -

Translational coupling of nasST expression in Azotobacter vinelandii prevents overexpression of the nasT gene

Abstract

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