Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary

TY - JOUR

T1 - Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary

AU - Couchman, John R

PY - 2019

Y1 - 2019

N2 - Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.

AB - Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.

KW - Faculty of Health and Medical Sciences

U2 - 10.5603/fhc.a2019.0017

DO - 10.5603/fhc.a2019.0017

M3 - Journal article

C2 - 31743418

SN - 0239-8508

JO - Folia Histochemica et Cytobiologica

JF - Folia Histochemica et Cytobiologica

ER -