Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary

John R Couchman

doi:10.5603/fhc.a2019.0017

Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary

Abstract

Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.

Originalsprog	Engelsk
Tidsskrift	Folia Histochemica et Cytobiologica
ISSN	0239-8508
DOI	https://doi.org/10.5603/fhc.a2019.0017
Status	Udgivet - 2019

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10.5603/fhc.a2019.0017

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AB - Transforming growth factor-β (TGF-β) is an important regulator of many cellular and immunological functions. It is often deposited in extracellular matrices in a latent form. This commentary is to draw attention to the likelihood that preparing cell-free matrices from tissue cultures by high pH buffers, such as ammonium hydroxide, can activate the TGF-β. Therefore, cells subsequently seeded onto such matrices may respond to the presence of active TGF-β in addition to interactions with macromolecular extracellular matrix components.

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Transforming growth factor-β activation in cell-free extracellular matrix preparations. Commentary

Abstract

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