Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

Maren Julia Pröll; Christiane Neuhoff; Karl Schellander; Muhammad Jasim Uddin; Mehmet Ulas Cinar; Sudeep Sahadevan; Xueqi Qu; Aminul Islam; Mikhael Poirier; Marcel A. Müller; Christian Drosten; Dawit Tesfaye; Ernst Tholen; Christine Große-Brinkhaus

doi:10.1371/journal.pone.0187735

Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

Maren Julia Pröll, Christiane Neuhoff^*, Karl Schellander, Muhammad Jasim Uddin, Mehmet Ulas Cinar, Sudeep Sahadevan, Xueqi Qu, Aminul Islam, Mikhael Poirier, Marcel A. Müller, Christian Drosten, Dawit Tesfaye, Ernst Tholen, Christine Große-Brinkhaus

^*Corresponding author af dette arbejde

11 Citationer (Scopus)

183 Downloads (Pure)

Abstract

The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.

Originalsprog	Engelsk
Artikelnummer	e0187735
Tidsskrift	PLoS ONE
Vol/bind	12
Udgave nummer	11
Antal sider	20
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0187735
Status	Udgivet - 1 nov. 2017

FN’s Verdensmål

Dette resultat bidrager til følgende verdensmål

Adgang til dokumentet

10.1371/journal.pone.0187735Licens: CC BY

Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infectionForlagets udgivne version, 1,73 MBLicens: CC BY

Citationsformater

Pröll, M. J., Neuhoff, C., Schellander, K., Uddin, M. J., Cinar, M. U., Sahadevan, S., Qu, X., Islam, A., Poirier, M., Müller, M. A., Drosten, C., Tesfaye, D., Tholen, E., & Große-Brinkhaus, C. (2017). Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection. PLoS ONE, 12(11), [e0187735]. https://doi.org/10.1371/journal.pone.0187735

Pröll, MJ, Neuhoff, C, Schellander, K, Uddin, MJ, Cinar, MU, Sahadevan, S, Qu, X, Islam, A, Poirier, M, Müller, MA, Drosten, C, Tesfaye, D, Tholen, E & Große-Brinkhaus, C 2017, 'Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection', PLoS ONE, bind 12, nr. 11, e0187735. https://doi.org/10.1371/journal.pone.0187735

@article{06d06199114b44d496d8844c5cb512b7,

title = "Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection",

abstract = "The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.",

author = "Pr{\"o}ll, {Maren Julia} and Christiane Neuhoff and Karl Schellander and Uddin, {Muhammad Jasim} and Cinar, {Mehmet Ulas} and Sudeep Sahadevan and Xueqi Qu and Aminul Islam and Mikhael Poirier and M{\"u}ller, {Marcel A.} and Christian Drosten and Dawit Tesfaye and Ernst Tholen and Christine Gro{\ss}e-Brinkhaus",

year = "2017",

month = nov,

day = "1",

doi = "10.1371/journal.pone.0187735",

language = "English",

volume = "12",

journal = "PLoS Computational Biology",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "11",

}

TY - JOUR

T1 - Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

AU - Pröll, Maren Julia

AU - Neuhoff, Christiane

AU - Schellander, Karl

AU - Uddin, Muhammad Jasim

AU - Cinar, Mehmet Ulas

AU - Sahadevan, Sudeep

AU - Qu, Xueqi

AU - Islam, Aminul

AU - Poirier, Mikhael

AU - Müller, Marcel A.

AU - Drosten, Christian

AU - Tesfaye, Dawit

AU - Tholen, Ernst

AU - Große-Brinkhaus, Christine

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.

AB - The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.

U2 - 10.1371/journal.pone.0187735

DO - 10.1371/journal.pone.0187735

M3 - Journal article

C2 - 29140992

AN - SCOPUS:85034094231

SN - 1932-6203

VL - 12

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 11

M1 - e0187735

ER -

Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

Abstract

FN’s Verdensmål

Adgang til dokumentet

Fingeraftryk

Citationsformater