TY - JOUR
T1 - Transcriptional profiling of five isolated size-matched stages of human preantral follicles
AU - Kristensen, Stine Gry
AU - Ebbesen, Pernille
AU - Andersen, Claus Yding
N1 - Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
PY - 2015/2/5
Y1 - 2015/2/5
N2 - Little is known of the early stages of human follicular development and the complex processes that regulate follicular growth. To identify genes of potential importance, we analysed follicle-related transcripts in five populations of isolated size-matched human preantral follicles by microarray analysis. Oocyte-specific genes were found to be the most abundant and differentially expressed transcripts and included germ cell transcription factors LHX8 and SOHLH2 which were significantly down-regulated during preantral follicle development. Differentially expressed genes also included transcription factors of NOTCH signalling, IGF2, orphan nuclear receptor LRH-1, and homeobox gene HOXA7, indicating potentially important regulatory roles for these genes during early human folliculogenesis. We also found that FSHR mRNA and protein were present in the earliest stages of preantral follicles, whereas LHR was absent. In conclusion, our data identify specific oocyte and somatic genes in small human follicles that impact early follicle growth, and provide foundation for further analysis of the signalling pathways involved in early human folliculogenesis.
AB - Little is known of the early stages of human follicular development and the complex processes that regulate follicular growth. To identify genes of potential importance, we analysed follicle-related transcripts in five populations of isolated size-matched human preantral follicles by microarray analysis. Oocyte-specific genes were found to be the most abundant and differentially expressed transcripts and included germ cell transcription factors LHX8 and SOHLH2 which were significantly down-regulated during preantral follicle development. Differentially expressed genes also included transcription factors of NOTCH signalling, IGF2, orphan nuclear receptor LRH-1, and homeobox gene HOXA7, indicating potentially important regulatory roles for these genes during early human folliculogenesis. We also found that FSHR mRNA and protein were present in the earliest stages of preantral follicles, whereas LHR was absent. In conclusion, our data identify specific oocyte and somatic genes in small human follicles that impact early follicle growth, and provide foundation for further analysis of the signalling pathways involved in early human folliculogenesis.
KW - Basic Helix-Loop-Helix Transcription Factors
KW - Female
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Developmental
KW - Humans
KW - Insulin-Like Growth Factor II
KW - LIM-Homeodomain Proteins
KW - MSX1 Transcription Factor
KW - Oligonucleotide Array Sequence Analysis
KW - Ovarian Follicle
KW - Receptors, Cytoplasmic and Nuclear
KW - Receptors, FSH
KW - Signal Transduction
KW - Transcription Factors
KW - Young Adult
U2 - 10.1016/j.mce.2014.12.012
DO - 10.1016/j.mce.2014.12.012
M3 - Journal article
C2 - 25528519
SN - 0303-7207
VL - 401
SP - 189
EP - 201
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
ER -