Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

Rohit Sachdeva; Marie E. Jönsson; Jenny Nelander; Agnete Kirkeby; Carolina Guibentif; Bernhard Gentner; Luigi Naldini; Anders Björklund; Malin Parmar; Johan Jakobsson

doi:10.1073/pnas.1006568107

Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

Rohit Sachdeva, Marie E. Jönsson, Jenny Nelander, Agnete Kirkeby, Carolina Guibentif, Bernhard Gentner, Luigi Naldini, Anders Björklund, Malin Parmar, Johan Jakobsson^*

^*Corresponding author af dette arbejde

Laboratory

31 Citationer (Scopus)

Abstract

In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they differentiated toward the neural lineage. In addition, this strategy was successfully used to FACS purify neuronal progenitors for molecular analysis and transplantation. FACS enrichment reduced tumor formation and increased survival of ES cell-derived neuronal progenitors after transplantation. The properties and versatility of the microRNA-regulated vectors allows broad use of these vectors in stem cell applications.

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Sciences of the United States of America
Vol/bind	107
Udgave nummer	25
Sider (fra-til)	11602-11607
Antal sider	6
ISSN	0027-8424
DOI	https://doi.org/10.1073/pnas.1006568107
Status	Udgivet - 22 jun. 2010

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Sachdeva, R., Jönsson, M. E., Nelander, J., Kirkeby, A., Guibentif, C., Gentner, B., Naldini, L., Björklund, A., Parmar, M., & Jakobsson, J. (2010). Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. Proceedings of the National Academy of Sciences of the United States of America, 107(25), 11602-11607. https://doi.org/10.1073/pnas.1006568107

Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors. / Sachdeva, Rohit; Jönsson, Marie E.; Nelander, Jenny et al.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 107, Nr. 25, 22.06.2010, s. 11602-11607.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Sachdeva, R, Jönsson, ME, Nelander, J, Kirkeby, A, Guibentif, C, Gentner, B, Naldini, L, Björklund, A, Parmar, M & Jakobsson, J 2010, 'Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors', Proceedings of the National Academy of Sciences of the United States of America, bind 107, nr. 25, s. 11602-11607. https://doi.org/10.1073/pnas.1006568107

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AU - Guibentif, Carolina

AU - Gentner, Bernhard

AU - Naldini, Luigi

AU - Björklund, Anders

AU - Parmar, Malin

AU - Jakobsson, Johan

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Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

Abstract

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