Tissue inhibitor of metalloproteinases-1 as a biological marker ?in colorectal cancer: influence of smoking on plasma levels?

TY - JOUR

T1 - Tissue inhibitor of metalloproteinases-1 as a biological marker ?in colorectal cancer

T2 - influence of smoking on plasma levels?

AU - Rasmussen, Louise

AU - Ladelund, Steen

AU - Brünner, Nils Aage

AU - Jørgensen, Lars N

AU - Nielsen, Hans Jørgen

AU - Sørensen, Lars T

PY - 2013/4

Y1 - 2013/4

N2 - Background: At present plasma tissue inhibitor of metalloproteinases-1 (TIMP-1) is undergoing validation as a biological marker in colorectal cancer (CRC). The clinical implementation of plasma TIMP-1 in prognosis, prediction, screening and monitoring CRC requires robust information as to the influence of preanalytical factors, including inter-and intrapersonal biological variations. The aim of the present study was to evaluate the possible effects of smoking on the level of TIMP-1 in plasma from healthy subjects. Materials and methods: Forty-six never-smokers and 48 daily smokers participated in the 13-week study. Smokers were randomized into 3 groups of 16 subjects each: one group continued to smoke, a second group refrained from smoking and used a transdermal nicotine patch, and a third group refrained from smoking and used placebo patches. Plasma TIMP-1 levels were determined using ELISA. Results: No significant differences in TIMP-1 levels could be shown between the smoking group, the 2 different abstinent smoking groups and the never-smoking group. Conclusion: Smoking does not appear to have any influence on TIMP-1 levels in plasma collected from healthy subjects.

AB - Background: At present plasma tissue inhibitor of metalloproteinases-1 (TIMP-1) is undergoing validation as a biological marker in colorectal cancer (CRC). The clinical implementation of plasma TIMP-1 in prognosis, prediction, screening and monitoring CRC requires robust information as to the influence of preanalytical factors, including inter-and intrapersonal biological variations. The aim of the present study was to evaluate the possible effects of smoking on the level of TIMP-1 in plasma from healthy subjects. Materials and methods: Forty-six never-smokers and 48 daily smokers participated in the 13-week study. Smokers were randomized into 3 groups of 16 subjects each: one group continued to smoke, a second group refrained from smoking and used a transdermal nicotine patch, and a third group refrained from smoking and used placebo patches. Plasma TIMP-1 levels were determined using ELISA. Results: No significant differences in TIMP-1 levels could be shown between the smoking group, the 2 different abstinent smoking groups and the never-smoking group. Conclusion: Smoking does not appear to have any influence on TIMP-1 levels in plasma collected from healthy subjects.

U2 - 10.5301/jbm.5000017

DO - 10.5301/jbm.5000017

M3 - Journal article

C2 - 23592002

SN - 0393-6155

VL - 28

SP - 226

EP - 230

JO - International Journal of Biological Markers

JF - International Journal of Biological Markers

IS - 2

ER -