The VEGF system and tie-2 are spatio-temporal expressed during tayassu placentation

TY - ABST

T1 - The VEGF system and tie-2 are spatio-temporal expressed during tayassu placentation

AU - Miglino, M.A.

AU - Santos, T.C.

AU - Papa, P.C.

AU - Oliveira, M.F.

AU - Dantzer, Vibeke

N1 - Serie: Placenta, Elsevier, 27, 9-10, 0143-4004 Sider: A.32

PY - 2006

Y1 - 2006

N2 - Objectives: The vascular endothelial growth factor (VEGF) is one of the most important vascular mitogens, while the angiotensin receptor Tie-2 binds to the angiopoietin and stabilizes newly formed vessels. We therefore wanted to localize VEGF and its receptors VEGF-R1, VEGF-R2 and the Tie-2 receptor during gestation in the placenta of the Tayassu tajacu (collared peccary) to compare with other epitheliochorial placentae. Methods: Tissues from 10 placentae (first, middle and late gestation), obtained from CEMAS, Brazil, were collected in 10% buffered formalin and processed by routine methods for immunhistochemistry using polyclonal antibodies against VEGF, VEGFR-1, VEGFR-2 and Tie-2. Results: In the present study the VEGF-system exhibited intense staining in the uterine epithelium, uterine glandular epithelium and trophoblast. The endothelial cells and smooth muscle cells of the vessels in the maternal and fetal compartment were also immunoreactive. Placentae from initial phase of gestation showed weaker staining when compared to middle and late gestation. In late gestation of the tayassu the Tie-2 was co-localized with the VEGF-system in both maternal (intense staining in glandular and uterine epithelium) and fetal tissues. These results are similar to that described in swine and bovine placentae suggesting a similar physiological role between these 2 major angiogenic families, VEGF and angiopoietins. Conclusion: The VEGF system plays an import role in vascularization of tayassu placenta from the beginning to the end of gestation and Tie-2 is needed to stabilize blood vessels also in late gestation.

AB - Objectives: The vascular endothelial growth factor (VEGF) is one of the most important vascular mitogens, while the angiotensin receptor Tie-2 binds to the angiopoietin and stabilizes newly formed vessels. We therefore wanted to localize VEGF and its receptors VEGF-R1, VEGF-R2 and the Tie-2 receptor during gestation in the placenta of the Tayassu tajacu (collared peccary) to compare with other epitheliochorial placentae. Methods: Tissues from 10 placentae (first, middle and late gestation), obtained from CEMAS, Brazil, were collected in 10% buffered formalin and processed by routine methods for immunhistochemistry using polyclonal antibodies against VEGF, VEGFR-1, VEGFR-2 and Tie-2. Results: In the present study the VEGF-system exhibited intense staining in the uterine epithelium, uterine glandular epithelium and trophoblast. The endothelial cells and smooth muscle cells of the vessels in the maternal and fetal compartment were also immunoreactive. Placentae from initial phase of gestation showed weaker staining when compared to middle and late gestation. In late gestation of the tayassu the Tie-2 was co-localized with the VEGF-system in both maternal (intense staining in glandular and uterine epithelium) and fetal tissues. These results are similar to that described in swine and bovine placentae suggesting a similar physiological role between these 2 major angiogenic families, VEGF and angiopoietins. Conclusion: The VEGF system plays an import role in vascularization of tayassu placenta from the beginning to the end of gestation and Tie-2 is needed to stabilize blood vessels also in late gestation.

M3 - Conference abstract for conference

T2 - IFPA 2006

Y2 - 29 November 2010

ER -