The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation

TY - JOUR

T1 - The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation

AU - Jacobsen, Stine

AU - Adler, Ditte Marie Top

AU - Bundgaard, Louise

AU - Sørensen, Mette Aamand

AU - Andersen, Pia Haubro

AU - Bendixen, Emøke

PY - 2014/12/1

Y1 - 2014/12/1

N2 - The objective of the study was to assess global expression of proteins in equine saliva using liquid chromatography tandem mass spectrometry (LC-MS/MS). Saliva was obtained from seven horses with and six horses without evidence of systemic inflammatory disease. Tryptic peptides from saliva were analysed by LC-MS/MS.Of 195 unique proteins identified, 57 were detected only in saliva samples from horses with systemic inflammation (in two to six of the seven horses). Among the differentially expressed proteins were several acute phase proteins (APPs) such as serum amyloid A, fibrinogen, haptoglobin, and alpha1-acid glycoprotein.The study is the first to describe detection of inflammatory proteins in horse saliva. The proteins detected were similar to those described in saliva from cattle, small ruminants and pigs. Detection of APPs in horses with systemic inflammation suggests that saliva may be used for non-invasive disease monitoring in horses as in humans, pigs and dogs.

AB - The objective of the study was to assess global expression of proteins in equine saliva using liquid chromatography tandem mass spectrometry (LC-MS/MS). Saliva was obtained from seven horses with and six horses without evidence of systemic inflammatory disease. Tryptic peptides from saliva were analysed by LC-MS/MS.Of 195 unique proteins identified, 57 were detected only in saliva samples from horses with systemic inflammation (in two to six of the seven horses). Among the differentially expressed proteins were several acute phase proteins (APPs) such as serum amyloid A, fibrinogen, haptoglobin, and alpha1-acid glycoprotein.The study is the first to describe detection of inflammatory proteins in horse saliva. The proteins detected were similar to those described in saliva from cattle, small ruminants and pigs. Detection of APPs in horses with systemic inflammation suggests that saliva may be used for non-invasive disease monitoring in horses as in humans, pigs and dogs.

U2 - 10.1016/j.tvjl.2014.08.032

DO - 10.1016/j.tvjl.2014.08.032

M3 - Journal article

C2 - 25296850

SN - 1090-0233

VL - 202

SP - 483

EP - 488

JO - Veterinary Journal

JF - Veterinary Journal

IS - 3

ER -