The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

Maria Alhede; Camilla Stavnsbjerg; Thomas Bjarnsholt

doi:10.1111/apm.12888

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

Maria Alhede^*, Camilla Stavnsbjerg, Thomas Bjarnsholt

^*Corresponding author af dette arbejde

2 Citationer (Scopus)

Abstract

The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

Originalsprog	Engelsk
Tidsskrift	APMIS
Vol/bind	126
Udgave nummer	10
Sider (fra-til)	779-794
Antal sider	16
ISSN	0903-4641
DOI	https://doi.org/10.1111/apm.12888
Status	Udgivet - 2018

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10.1111/apm.12888

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title = "The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria",

abstract = "The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.",

keywords = "chronic bacterial infections, confocal scanning microscope, fluorescent staining, Morphology, polymorphonuclear leukocytes",

author = "Maria Alhede and Camilla Stavnsbjerg and Thomas Bjarnsholt",

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doi = "10.1111/apm.12888",

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AU - Alhede, Maria

AU - Stavnsbjerg, Camilla

AU - Bjarnsholt, Thomas

PY - 2018

Y1 - 2018

N2 - The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

AB - The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

KW - chronic bacterial infections

KW - confocal scanning microscope

KW - fluorescent staining

KW - Morphology

KW - polymorphonuclear leukocytes

U2 - 10.1111/apm.12888

DO - 10.1111/apm.12888

M3 - Review

C2 - 30191615

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SP - 779

EP - 794

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 10

ER -

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

Abstract

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