The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

L H Engelholm; B S Nielsen; Sarah Netzel-Arnett; H. Solberg; X D Chen; J M Lopez Garcia; Carlos López-Otín; M F Young; H. Birkedal-Hansen; K Danø; L R Lund; N Behrendt; T H Bugge

The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

L H Engelholm, B S Nielsen, Sarah Netzel-Arnett, H. Solberg, X D Chen, J M Lopez Garcia, Carlos López-Otín, M F Young, H. Birkedal-Hansen, K Danø, L R Lund, N Behrendt, T H Bugge

59 Citationer (Scopus)

Abstract

The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

Originalsprog	Engelsk
Tidsskrift	Laboratory Investigation
Vol/bind	81
Udgave nummer	10
Sider (fra-til)	1403-14
Antal sider	12
ISSN	0023-6837
Status	Udgivet - okt. 2001

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Citationsformater

Engelholm, L. H., Nielsen, B. S., Netzel-Arnett, S., Solberg, H., Chen, X. D., Lopez Garcia, J. M., López-Otín, C., Young, M. F., Birkedal-Hansen, H., Danø, K., Lund, L. R., Behrendt, N., & Bugge, T. H. (2001). The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis. Laboratory Investigation, 81(10), 1403-14.

The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis. / Engelholm, L H; Nielsen, B S; Netzel-Arnett, Sarah et al.

I: Laboratory Investigation, Bind 81, Nr. 10, 10.2001, s. 1403-14.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Engelholm, LH, Nielsen, BS, Netzel-Arnett, S, Solberg, H, Chen, XD, Lopez Garcia, JM, López-Otín, C, Young, MF, Birkedal-Hansen, H, Danø, K, Lund, LR, Behrendt, N & Bugge, TH 2001, 'The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis', Laboratory Investigation, bind 81, nr. 10, s. 1403-14.

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title = "The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis",

abstract = "The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.",

keywords = "Animals, Bone and Bones, Collagenases, Embryonic and Fetal Development, Female, Immunohistochemistry, Matrix Metalloproteinase 13, Mice, Osteogenesis, Pregnancy, Receptors, Cell Surface, Receptors, Mitogen, Receptors, Urokinase Plasminogen Activator, Journal Article, Research Support, Non-U.S. Gov't",

author = "Engelholm, {L H} and Nielsen, {B S} and Sarah Netzel-Arnett and H. Solberg and Chen, {X D} and {Lopez Garcia}, {J M} and Carlos L{\'o}pez-Ot{\'i}n and Young, {M F} and H. Birkedal-Hansen and K Dan{\o} and Lund, {L R} and N Behrendt and Bugge, {T H}",

year = "2001",

month = oct,

language = "English",

volume = "81",

pages = "1403--14",

journal = "Laboratory Investigation",

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number = "10",

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TY - JOUR

T1 - The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

AU - Engelholm, L H

AU - Nielsen, B S

AU - Netzel-Arnett, Sarah

AU - Solberg, H.

AU - Chen, X D

AU - Lopez Garcia, J M

AU - López-Otín, Carlos

AU - Young, M F

AU - Birkedal-Hansen, H.

AU - Danø, K

AU - Lund, L R

AU - Behrendt, N

AU - Bugge, T H

PY - 2001/10

Y1 - 2001/10

N2 - The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

AB - The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.

KW - Animals

KW - Bone and Bones

KW - Collagenases

KW - Embryonic and Fetal Development

KW - Female

KW - Immunohistochemistry

KW - Matrix Metalloproteinase 13

KW - Mice

KW - Osteogenesis

KW - Pregnancy

KW - Receptors, Cell Surface

KW - Receptors, Mitogen

KW - Receptors, Urokinase Plasminogen Activator

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 11598153

SN - 0023-6837

VL - 81

SP - 1403

EP - 1414

JO - Laboratory Investigation

JF - Laboratory Investigation

IS - 10

ER -