The triglyceride content in skeletal muscle is associated with hepatic but not peripheral insulin resistance in elderly twins

TY - JOUR

T1 - The triglyceride content in skeletal muscle is associated with hepatic but not peripheral insulin resistance in elderly twins

AU - Grunnet, L G

AU - Laurila, Esa

AU - Hansson, Ola

AU - Almgren, Peter

AU - Groop, Leif

AU - Brøns, Charlotte

AU - Poulsen, Pernille Hartveit

AU - Vaag, Allan

PY - 2012/12

Y1 - 2012/12

N2 - Context and Objective: Total muscle triglyceride (MT) content has been associated with insulin resistance. We investigated the predictors and impact of MT on relevant metabolic parameters including peripheral and hepatic insulin resistance in elderly twins. Design and Participants: Seventy-four elderly same-sex twins underwent hyperinsulinemic euglycemic clamps preceded by an iv glucose tolerance test. Aerobic capacity (VO2max) and body composition (dual-energy x-ray absorptiometry scan) were determined in all twins. A biopsy from the vastus lateralis muscle was excised in the fasting state. The muscle triacylglycerol content was analyzed by biochemical extraction from these biopsies. Results: The percentage of total body fat was the only independent predictor of MT content. After adjustment for trunk fat percentages and sex, MT level was significantly associated to fasting plasma levels of glucose and insulin as well as hepatic insulin resistance. However, the association wasweakenedafter adjustment for total fat percentages.A1 SD (34.5mmol/kgdry weight) increase in MT content was associated with a 24% increase of hepatic insulin resistance. No association between MT content and peripheral insulin sensitivity was observed. Conclusion: MT content is associated with hepatic but not peripheral insulin resistance in elderly twins. Wespeculate that MT content may reflect the general ectopic accumulation of triglycerides, including fat in the liver.

AB - Context and Objective: Total muscle triglyceride (MT) content has been associated with insulin resistance. We investigated the predictors and impact of MT on relevant metabolic parameters including peripheral and hepatic insulin resistance in elderly twins. Design and Participants: Seventy-four elderly same-sex twins underwent hyperinsulinemic euglycemic clamps preceded by an iv glucose tolerance test. Aerobic capacity (VO2max) and body composition (dual-energy x-ray absorptiometry scan) were determined in all twins. A biopsy from the vastus lateralis muscle was excised in the fasting state. The muscle triacylglycerol content was analyzed by biochemical extraction from these biopsies. Results: The percentage of total body fat was the only independent predictor of MT content. After adjustment for trunk fat percentages and sex, MT level was significantly associated to fasting plasma levels of glucose and insulin as well as hepatic insulin resistance. However, the association wasweakenedafter adjustment for total fat percentages.A1 SD (34.5mmol/kgdry weight) increase in MT content was associated with a 24% increase of hepatic insulin resistance. No association between MT content and peripheral insulin sensitivity was observed. Conclusion: MT content is associated with hepatic but not peripheral insulin resistance in elderly twins. Wespeculate that MT content may reflect the general ectopic accumulation of triglycerides, including fat in the liver.

U2 - 10.1210/jc.2012-2061

DO - 10.1210/jc.2012-2061

M3 - Journal article

C2 - 23055544

SN - 0021-972X

VL - 97

SP - 4571

EP - 4577

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 12

ER -