The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion

TY - JOUR

T1 - The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion

AU - Styrkársdóttir, U

AU - Egel, R

AU - Nielsen, O

AU - Nielsen, Olaf

PY - 1993/2/1

Y1 - 1993/2/1

N2 - Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of the H1 homology box, 31 bp from the site of the DSB, and extends into the flanking region distal to mat1. The sequence of the region distal to H1 in the wild-type is also presented. In this region we observe a bias in the distribution of purine residues between the two DNA strands.

AB - Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of the H1 homology box, 31 bp from the site of the DSB, and extends into the flanking region distal to mat1. The sequence of the region distal to H1 in the wild-type is also presented. In this region we observe a bias in the distribution of purine residues between the two DNA strands.

KW - Base Sequence

KW - DNA, Fungal

KW - DNA, Recombinant

KW - Genes, Fungal

KW - Genes, Mating Type, Fungal

KW - Molecular Sequence Data

KW - Mutation

KW - Schizosaccharomyces

KW - Sequence Deletion

M3 - Journal article

C2 - 8431959

SN - 0172-8083

VL - 23

SP - 184

EP - 186

JO - Current Genetics

JF - Current Genetics

IS - 2

ER -