The Sertoli cell hormones inhibin-B and anti Müllerian hormone have different patterns of secretion in prepubertal cryptorchid boys

TY - JOUR

T1 - The Sertoli cell hormones inhibin-B and anti Müllerian hormone have different patterns of secretion in prepubertal cryptorchid boys

AU - Cortes, Dina

AU - Clasen-Linde, Erik

AU - Hutson, John M

AU - Li, Ruili

AU - Thorup, Jorgen

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Objectives and hypotheses The Sertoli-cells produce inhibin-B and Anti-Müllerian-Hormone (AMH). Much is still unknown about these hormones in prepubertal cryptorchids. The Sertoli-cells are mandatory for germ cell development. The aim of the study was to investigate if there are differences in secretion pattern of Sertoli-cell hormones and their gonadotropin feed-back mechanisms. Methods Included were 94 prepubertal cryptorchid boys 0.5-13.1 years with measurements of serum-inhibin-B, Anti-Müllerian-Hormone (AMH), Luteinizing Hormone (LH) and Follicle Stimulation Hormone (FSH). The serum values were measured using commercially available kits. The hormonal values were related to age-matched normal values. Testicular biopsy was taken at orchiopexy. Results Inhibin-B positively correlated to AMH for 1-13 year-old patients (p < 0.0001), but not for 0.5-1 year-old patients (p = 0.439). For 0.5-1 year-old patients inhibin-B-values tended to decrease (p = 0.055), in contrast to AMH-values (p = 0.852). LH was elevated more often than FSH (p = 0.014). FSH and LH were positively associated in patients both 0.5-1 year (p = 0.042) and 1-13 years of age (p < 0.0001). LH correlated positively to inhibin- B (p = 0.001). In contrast, FSH did not correlate to inhibin-B or AMH (p = 0.755 and p = 0.528). The number of A-dark spermatogonia per tubular transverse section was positively correlated to inhibin-B serum level. Conclusion Our new finding of an association between LH and inhibin-B in infancy of cryptorchid boys may be essential for the transformation of gonocytes to A-dark spermatogonia. Previously, LH associated to inhibin-B was described in early puberty only. During the first year of life inhibin-B values decreased faster than AMH. The AMH-levels may just reflect the increased Sertoli cell number that occurs during the first 3 months of life.

AB - Objectives and hypotheses The Sertoli-cells produce inhibin-B and Anti-Müllerian-Hormone (AMH). Much is still unknown about these hormones in prepubertal cryptorchids. The Sertoli-cells are mandatory for germ cell development. The aim of the study was to investigate if there are differences in secretion pattern of Sertoli-cell hormones and their gonadotropin feed-back mechanisms. Methods Included were 94 prepubertal cryptorchid boys 0.5-13.1 years with measurements of serum-inhibin-B, Anti-Müllerian-Hormone (AMH), Luteinizing Hormone (LH) and Follicle Stimulation Hormone (FSH). The serum values were measured using commercially available kits. The hormonal values were related to age-matched normal values. Testicular biopsy was taken at orchiopexy. Results Inhibin-B positively correlated to AMH for 1-13 year-old patients (p < 0.0001), but not for 0.5-1 year-old patients (p = 0.439). For 0.5-1 year-old patients inhibin-B-values tended to decrease (p = 0.055), in contrast to AMH-values (p = 0.852). LH was elevated more often than FSH (p = 0.014). FSH and LH were positively associated in patients both 0.5-1 year (p = 0.042) and 1-13 years of age (p < 0.0001). LH correlated positively to inhibin- B (p = 0.001). In contrast, FSH did not correlate to inhibin-B or AMH (p = 0.755 and p = 0.528). The number of A-dark spermatogonia per tubular transverse section was positively correlated to inhibin-B serum level. Conclusion Our new finding of an association between LH and inhibin-B in infancy of cryptorchid boys may be essential for the transformation of gonocytes to A-dark spermatogonia. Previously, LH associated to inhibin-B was described in early puberty only. During the first year of life inhibin-B values decreased faster than AMH. The AMH-levels may just reflect the increased Sertoli cell number that occurs during the first 3 months of life.

KW - Journal Article

U2 - 10.1016/j.jpedsurg.2015.08.059

DO - 10.1016/j.jpedsurg.2015.08.059

M3 - Journal article

C2 - 26452703

SN - 0022-3468

VL - 51

SP - 475

EP - 480

JO - Journal of Pediatric Surgery

JF - Journal of Pediatric Surgery

IS - 3

ER -