The Schizosaccharomyces pombe map1 gene encodes an SRF/MCM1-related protein required for P-cell specific gene expression

TY - JOUR

T1 - The Schizosaccharomyces pombe map1 gene encodes an SRF/MCM1-related protein required for P-cell specific gene expression

AU - Nielsen, O

AU - Friis, T

AU - Kjaerulff, S

AU - Nielsen, Olaf

PY - 1996/12/13

Y1 - 1996/12/13

N2 - Cells of Schizosaccharomyces pombe undergo mating and meiosis when starved for a nitrogen source. In this process a P and and M cell first mate to generate a diploid zygote, which subsequently enters meiosis and sporulates. The P mating type is controlled by the mat1-Pc gene at the mating type locus, together with a gene called map1. We show that these two functions are required for expression of the P-specific gene map3. We have cloned the map1 gene and show that it encodes a putative MADS-box containing transcription factor related to mammalian Serum Response Factor (SRF) and Saccharomyces cerevisiae MCM1. The Mat1-Pc protein contains a motif characteristic for proteins that interact with MADS-box factors, suggesting that Mat-Pc and Map1 may form a heterodimer that activates the P-specific map3 gene.

AB - Cells of Schizosaccharomyces pombe undergo mating and meiosis when starved for a nitrogen source. In this process a P and and M cell first mate to generate a diploid zygote, which subsequently enters meiosis and sporulates. The P mating type is controlled by the mat1-Pc gene at the mating type locus, together with a gene called map1. We show that these two functions are required for expression of the P-specific gene map3. We have cloned the map1 gene and show that it encodes a putative MADS-box containing transcription factor related to mammalian Serum Response Factor (SRF) and Saccharomyces cerevisiae MCM1. The Mat1-Pc protein contains a motif characteristic for proteins that interact with MADS-box factors, suggesting that Mat-Pc and Map1 may form a heterodimer that activates the P-specific map3 gene.

KW - Amino Acid Sequence

KW - Cloning, Molecular

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Fungal

KW - Genes, Fungal

KW - Genes, Mating Type, Fungal

KW - High Mobility Group Proteins

KW - Homeodomain Proteins

KW - MCM1 Protein

KW - Molecular Sequence Data

KW - Nuclear Proteins

KW - Restriction Mapping

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Homology, Amino Acid

KW - Serum Response Factor

KW - Transcription Factors

M3 - Journal article

C2 - 9003326

SN - 0026-8925

VL - 253

SP - 387

EP - 392

JO - Molecular and General Genetics MGG

JF - Molecular and General Genetics MGG

IS - 3

ER -