The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

T. Borgbo; J. Chrudimska; M. Macek; J. V. Jeppesen; J. A. Bøtkjær; S. G. Kristensen; K. T. Macklon; E. Ernst; L. L. Hansen; C. Yding Andersen

doi:10.1016/j.mce.2017.07.002

The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

T. Borgbo, J. Chrudimska, M. Macek, J. V. Jeppesen, J. A. Bøtkjær, S. G. Kristensen, K. T. Macklon, E. Ernst, L. L. Hansen, C. Yding Andersen^*

^*Corresponding author af dette arbejde

Institut for Klinisk Medicin

1 Citationer (Scopus)

Abstract

The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to –/insLQ and the −/− genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in –/insLQ compared to the −/− genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.

Originalsprog	Engelsk
Tidsskrift	Molecular and Cellular Endocrinology
Vol/bind	460
Sider (fra-til)	57-62
Antal sider	6
ISSN	0303-7207
DOI	https://doi.org/10.1016/j.mce.2017.07.002
Status	Udgivet - 15 jan. 2018

Adgang til dokumentet

10.1016/j.mce.2017.07.002

Citationsformater

Borgbo, T., Chrudimska, J., Macek, M., Jeppesen, J. V., Bøtkjær, J. A., Kristensen, S. G., Macklon, K. T., Ernst, E., Hansen, L. L., & Yding Andersen, C. (2018). The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles. Molecular and Cellular Endocrinology, 460, 57-62. https://doi.org/10.1016/j.mce.2017.07.002

The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles. / Borgbo, T.; Chrudimska, J.; Macek, M. et al.
I: Molecular and Cellular Endocrinology, Bind 460, 15.01.2018, s. 57-62.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Borgbo, T, Chrudimska, J, Macek, M, Jeppesen, JV, Bøtkjær, JA, Kristensen, SG, Macklon, KT, Ernst, E, Hansen, LL & Yding Andersen, C 2018, 'The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles', Molecular and Cellular Endocrinology, bind 460, s. 57-62. https://doi.org/10.1016/j.mce.2017.07.002

Borgbo T, Chrudimska J, Macek M, Jeppesen JV, Bøtkjær JA, Kristensen SG et al. The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles. Molecular and Cellular Endocrinology. 2018 jan. 15;460:57-62. doi: 10.1016/j.mce.2017.07.002

@article{c775997c94cf4730ad54ec9c7e6310e3,

title = "The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles",

abstract = "The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-M{\"u}llerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to –/insLQ and the −/− genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in –/insLQ compared to the −/− genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.",

keywords = "Follicle fluid, Human small antral follicles, LHR, rs4539842",

author = "T. Borgbo and J. Chrudimska and M. Macek and Jeppesen, {J. V.} and B{\o}tkj{\ae}r, {J. A.} and Kristensen, {S. G.} and Macklon, {K. T.} and E. Ernst and Hansen, {L. L.} and {Yding Andersen}, C.",

year = "2018",

month = jan,

day = "15",

doi = "10.1016/j.mce.2017.07.002",

language = "English",

volume = "460",

pages = "57--62",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

AU - Borgbo, T.

AU - Chrudimska, J.

AU - Macek, M.

AU - Jeppesen, J. V.

AU - Bøtkjær, J. A.

AU - Kristensen, S. G.

AU - Macklon, K. T.

AU - Ernst, E.

AU - Hansen, L. L.

AU - Yding Andersen, C.

PY - 2018/1/15

Y1 - 2018/1/15

N2 - The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to –/insLQ and the −/− genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in –/insLQ compared to the −/− genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.

AB - The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to –/insLQ and the −/− genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in –/insLQ compared to the −/− genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.

KW - Follicle fluid

KW - Human small antral follicles

KW - LHR

KW - rs4539842

U2 - 10.1016/j.mce.2017.07.002

DO - 10.1016/j.mce.2017.07.002

M3 - Journal article

C2 - 28684292

AN - SCOPUS:85024474722

SN - 0303-7207

VL - 460

SP - 57

EP - 62

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

ER -

The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater