The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment

Lena Björkman; Jonas Mårtensson; Malene Winther; Michael Gabl; André Holdfeldt; Martin Uhrbom; Johan Bylund; Anders Højgaard Hansen; Sunil K Pandey; Trond Ulven; Huamei Forsman; Claes Dahlgren

doi:10.1128/mcb.00161-16

The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment

Lena Björkman, Jonas Mårtensson, Malene Winther, Michael Gabl, André Holdfeldt, Martin Uhrbom, Johan Bylund, Anders Højgaard Hansen, Sunil K Pandey, Trond Ulven, Huamei Forsman, Claes Dahlgren

21 Citationer (Scopus)

Abstract

Ligands with improved potency and selectivity for free fatty acid receptor 2 (FFA2R) have become available, and we here characterize the neutrophil responses induced by one such agonist (Cmp1) and one antagonist (CATPB). Cmp1 triggered an increase in the cytosolic concentration of Ca(2+), and the neutrophils were then desensitized to Cmp1 and to acetate, a naturally occurring FFA2R agonist. The antagonist CATPB selectively inhibited responses induced by Cmp1 or acetate. The activated FFA2R induced superoxide anion secretion at a low level in naive blood neutrophils. This response was largely increased by tumor necrosis factor alpha (TNF-α) in a process associated with a recruitment of easily mobilizable granules, but neutrophils recruited to an aseptic inflammation in vivo were nonresponding. Superoxide production induced by Cmp1 was increased in latrunculin A-treated neutrophils, but no reactivation of desensitized FFA2R was induced by this drug, suggesting that the cytoskeleton is not directly involved in terminating the response. The functional and regulatory differences between the receptors that recognize short-chain fatty acids and formylated peptides, respectively, imply different roles of these receptors in the orchestration of inflammation and confirm the usefulness of a selective FFA2R agonist and antagonist as tools for the exploration of the precise role of the FFA2R.

Originalsprog	Engelsk
Tidsskrift	Molecular and Cellular Biology
Vol/bind	36
Udgave nummer	20
Sider (fra-til)	2583-2595
Antal sider	13
ISSN	0270-7306
DOI	https://doi.org/10.1128/mcb.00161-16
Status	Udgivet - 2016
Udgivet eksternt	Ja

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10.1128/mcb.00161-16

https://mcb.asm.org/content/mcb/36/20/2583.full.pdf

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Citationsformater

Björkman, L., Mårtensson, J., Winther, M., Gabl, M., Holdfeldt, A., Uhrbom, M., Bylund, J., Højgaard Hansen, A., Pandey, S. K., Ulven, T., Forsman, H., & Dahlgren, C. (2016). The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment. Molecular and Cellular Biology, 36(20), 2583-2595. https://doi.org/10.1128/mcb.00161-16

The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment. / Björkman, Lena; Mårtensson, Jonas; Winther, Malene et al.

I: Molecular and Cellular Biology, Bind 36, Nr. 20, 2016, s. 2583-2595.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Björkman, L, Mårtensson, J, Winther, M, Gabl, M, Holdfeldt, A, Uhrbom, M, Bylund, J, Højgaard Hansen, A, Pandey, SK, Ulven, T, Forsman, H & Dahlgren, C 2016, 'The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment', Molecular and Cellular Biology, bind 36, nr. 20, s. 2583-2595. https://doi.org/10.1128/mcb.00161-16

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title = "The Neutrophil Response Induced by an Agonist for Free Fatty Acid Receptor 2 (GPR43) Is Primed by Tumor Necrosis Factor Alpha and by Receptor Uncoupling from the Cytoskeleton but Attenuated by Tissue Recruitment",

abstract = "Ligands with improved potency and selectivity for free fatty acid receptor 2 (FFA2R) have become available, and we here characterize the neutrophil responses induced by one such agonist (Cmp1) and one antagonist (CATPB). Cmp1 triggered an increase in the cytosolic concentration of Ca(2+), and the neutrophils were then desensitized to Cmp1 and to acetate, a naturally occurring FFA2R agonist. The antagonist CATPB selectively inhibited responses induced by Cmp1 or acetate. The activated FFA2R induced superoxide anion secretion at a low level in naive blood neutrophils. This response was largely increased by tumor necrosis factor alpha (TNF-α) in a process associated with a recruitment of easily mobilizable granules, but neutrophils recruited to an aseptic inflammation in vivo were nonresponding. Superoxide production induced by Cmp1 was increased in latrunculin A-treated neutrophils, but no reactivation of desensitized FFA2R was induced by this drug, suggesting that the cytoskeleton is not directly involved in terminating the response. The functional and regulatory differences between the receptors that recognize short-chain fatty acids and formylated peptides, respectively, imply different roles of these receptors in the orchestration of inflammation and confirm the usefulness of a selective FFA2R agonist and antagonist as tools for the exploration of the precise role of the FFA2R.",

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AU - Björkman, Lena

AU - Mårtensson, Jonas

AU - Winther, Malene

AU - Gabl, Michael

AU - Holdfeldt, André

AU - Uhrbom, Martin

AU - Bylund, Johan

AU - Højgaard Hansen, Anders

AU - Pandey, Sunil K

AU - Ulven, Trond

AU - Forsman, Huamei

AU - Dahlgren, Claes

PY - 2016

Y1 - 2016

N2 - Ligands with improved potency and selectivity for free fatty acid receptor 2 (FFA2R) have become available, and we here characterize the neutrophil responses induced by one such agonist (Cmp1) and one antagonist (CATPB). Cmp1 triggered an increase in the cytosolic concentration of Ca(2+), and the neutrophils were then desensitized to Cmp1 and to acetate, a naturally occurring FFA2R agonist. The antagonist CATPB selectively inhibited responses induced by Cmp1 or acetate. The activated FFA2R induced superoxide anion secretion at a low level in naive blood neutrophils. This response was largely increased by tumor necrosis factor alpha (TNF-α) in a process associated with a recruitment of easily mobilizable granules, but neutrophils recruited to an aseptic inflammation in vivo were nonresponding. Superoxide production induced by Cmp1 was increased in latrunculin A-treated neutrophils, but no reactivation of desensitized FFA2R was induced by this drug, suggesting that the cytoskeleton is not directly involved in terminating the response. The functional and regulatory differences between the receptors that recognize short-chain fatty acids and formylated peptides, respectively, imply different roles of these receptors in the orchestration of inflammation and confirm the usefulness of a selective FFA2R agonist and antagonist as tools for the exploration of the precise role of the FFA2R.

AB - Ligands with improved potency and selectivity for free fatty acid receptor 2 (FFA2R) have become available, and we here characterize the neutrophil responses induced by one such agonist (Cmp1) and one antagonist (CATPB). Cmp1 triggered an increase in the cytosolic concentration of Ca(2+), and the neutrophils were then desensitized to Cmp1 and to acetate, a naturally occurring FFA2R agonist. The antagonist CATPB selectively inhibited responses induced by Cmp1 or acetate. The activated FFA2R induced superoxide anion secretion at a low level in naive blood neutrophils. This response was largely increased by tumor necrosis factor alpha (TNF-α) in a process associated with a recruitment of easily mobilizable granules, but neutrophils recruited to an aseptic inflammation in vivo were nonresponding. Superoxide production induced by Cmp1 was increased in latrunculin A-treated neutrophils, but no reactivation of desensitized FFA2R was induced by this drug, suggesting that the cytoskeleton is not directly involved in terminating the response. The functional and regulatory differences between the receptors that recognize short-chain fatty acids and formylated peptides, respectively, imply different roles of these receptors in the orchestration of inflammation and confirm the usefulness of a selective FFA2R agonist and antagonist as tools for the exploration of the precise role of the FFA2R.

U2 - 10.1128/mcb.00161-16

DO - 10.1128/mcb.00161-16

M3 - Journal article

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