The major diversification of Vγ1.1⁺ and Vγ2⁺ thymocytes in mice occurs after commitment to the γδ T-cell lineage

TY - JOUR

T1 - The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage

AU - Buus, Terkild B.

AU - Geisler, Carsten

AU - Lauritsen, Jens Peter H

PY - 2016/10/11

Y1 - 2016/10/11

N2 - γδ T cells are a heterogeneous cell population with different subsets playing specialized and often opposing roles during immune responses. A key question is whether γδ thymocytes are determined for their effector function already at an early stage, before their commitment to the γδ T-cell lineage, or are instructed during their later development. Here, we show that the adult Vγ1.1+ and Vγ2+ γδ T-cell subsets both go through a CD73+CD24+ development stage, and that the gene regulation involved in lineage commitment is shared by both subsets. We demonstrate that the major subset diversification first occurs after the cells have committed to the γδ T-cell lineage, strongly supporting an instructive model for functional programming of γδ T cells. In conclusion, we show that the two major adult γδ T-cell subsets in mice develop through a shared pathway utilizing similar cellular machinery and that they diverge after the CD24+CD73+ maturity stage.

AB - γδ T cells are a heterogeneous cell population with different subsets playing specialized and often opposing roles during immune responses. A key question is whether γδ thymocytes are determined for their effector function already at an early stage, before their commitment to the γδ T-cell lineage, or are instructed during their later development. Here, we show that the adult Vγ1.1+ and Vγ2+ γδ T-cell subsets both go through a CD73+CD24+ development stage, and that the gene regulation involved in lineage commitment is shared by both subsets. We demonstrate that the major subset diversification first occurs after the cells have committed to the γδ T-cell lineage, strongly supporting an instructive model for functional programming of γδ T cells. In conclusion, we show that the two major adult γδ T-cell subsets in mice develop through a shared pathway utilizing similar cellular machinery and that they diverge after the CD24+CD73+ maturity stage.

KW - CD73

KW - Cell differentiation

KW - Effector programming

KW - T-cell development

KW - γδ T cells

U2 - 10.1002/eji.201646407

DO - 10.1002/eji.201646407

M3 - Journal article

C2 - 27418188

AN - SCOPUS:84990862125

SN - 0014-2980

VL - 46

SP - 2363

EP - 2375

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 10

ER -