The influence of inflammation and hematocrit on clot strength in canine thromboelastographic hypercoagulability

Clara B. Marschner; Bo Wiinberg; Inge Tarnow; Bo Markussen; Line Kühnel; Louise Bochsen; Annemarie T. Kristensen

doi:10.1111/vec.12675

The influence of inflammation and hematocrit on clot strength in canine thromboelastographic hypercoagulability

Clara B. Marschner^*, Bo Wiinberg, Inge Tarnow, Bo Markussen, Line Kühnel, Louise Bochsen, Annemarie T. Kristensen

^*Corresponding author af dette arbejde

Institut for Matematiske Fag

8 Citationer (Scopus)

Abstract

Objective: To investigate parameters causing canine thromboelastographic hypercoagulability and to investigate whether thromboelastography (TEG) with Cytochalasin D (Cyt D) added is related to parameters of platelet activity. Design: Prospective observational study on hemostatic and inflammatory parameters. Data were collected between November 2012 and July 2013. Setting: University teaching hospital. Animals: Twenty-eight dogs suffering from diseases predisposing to thrombosis and 19 clinically healthy dogs. Diseased dogs were enrolled if they fulfilled inclusion criteria regarding age, size, informed client consent, and obtained a diagnosis of a disease that has been associated with thrombosis or hypercoagulability. Interventions: None. Measurements and Main Results: Parameters of coagulation and anticoagulation, fibrinolysis, and antifibrinolysis, platelet activity, inflammation, platelet count, and hematocrit were measured using CBC, TEG, platelet aggregation on multiplate, platelet activity on flow cytometry, and hemostatic and inflammatory markers on plasma and serum analyses. ANOVA and multilinear regression analyses indicated that especially hematocrit and the inflammatory parameters C-reactive protein and interleukin-8 showed best association with overall clot strength in diseased dogs with hypercoagulable TEG tracings. Ratios presumed to reflect platelet contribution to the TEG tracing obtained in TEG analyses with Cyt D were related especially with hematocrit and P-selectin expression of platelets measured after γ-Thrombin activation on flow cytometry. Conclusion: Overall clot strength in TEG analyses of the hypercoagulable dogs included in the present study appears to be primarily associated with inflammation as well as hematocrit. Furthermore, the ratio between standard TEG analyses and TEG analyses with Cyt D may reflect some degree of platelet activity.

Originalsprog	Engelsk
Tidsskrift	Journal of Veterinary Emergency and Critical Care
Vol/bind	28
Udgave nummer	1
Sider (fra-til)	20-30
Antal sider	11
ISSN	1476-4431
DOI	https://doi.org/10.1111/vec.12675
Status	Udgivet - 2018

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10.1111/vec.12675

Citationsformater

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title = "The influence of inflammation and hematocrit on clot strength in canine thromboelastographic hypercoagulability",

abstract = "Objective: To investigate parameters causing canine thromboelastographic hypercoagulability and to investigate whether thromboelastography (TEG) with Cytochalasin D (Cyt D) added is related to parameters of platelet activity. Design: Prospective observational study on hemostatic and inflammatory parameters. Data were collected between November 2012 and July 2013. Setting: University teaching hospital. Animals: Twenty-eight dogs suffering from diseases predisposing to thrombosis and 19 clinically healthy dogs. Diseased dogs were enrolled if they fulfilled inclusion criteria regarding age, size, informed client consent, and obtained a diagnosis of a disease that has been associated with thrombosis or hypercoagulability. Interventions: None. Measurements and Main Results: Parameters of coagulation and anticoagulation, fibrinolysis, and antifibrinolysis, platelet activity, inflammation, platelet count, and hematocrit were measured using CBC, TEG, platelet aggregation on multiplate, platelet activity on flow cytometry, and hemostatic and inflammatory markers on plasma and serum analyses. ANOVA and multilinear regression analyses indicated that especially hematocrit and the inflammatory parameters C-reactive protein and interleukin-8 showed best association with overall clot strength in diseased dogs with hypercoagulable TEG tracings. Ratios presumed to reflect platelet contribution to the TEG tracing obtained in TEG analyses with Cyt D were related especially with hematocrit and P-selectin expression of platelets measured after γ-Thrombin activation on flow cytometry. Conclusion: Overall clot strength in TEG analyses of the hypercoagulable dogs included in the present study appears to be primarily associated with inflammation as well as hematocrit. Furthermore, the ratio between standard TEG analyses and TEG analyses with Cyt D may reflect some degree of platelet activity.",

keywords = "Cytochalasin D, dog, platelet activity, TEG, thromboelastography, thrombosis",

author = "Marschner, {Clara B.} and Bo Wiinberg and Inge Tarnow and Bo Markussen and Line K{\"u}hnel and Louise Bochsen and Kristensen, {Annemarie T.}",

year = "2018",

doi = "10.1111/vec.12675",

language = "English",

volume = "28",

pages = "20--30",

journal = "Journal of Veterinary Emergency and Critical Care (Online Edition)",

issn = "1476-4431",

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TY - JOUR

T1 - The influence of inflammation and hematocrit on clot strength in canine thromboelastographic hypercoagulability

AU - Marschner, Clara B.

AU - Wiinberg, Bo

AU - Tarnow, Inge

AU - Markussen, Bo

AU - Kühnel, Line

AU - Bochsen, Louise

AU - Kristensen, Annemarie T.

PY - 2018

Y1 - 2018

N2 - Objective: To investigate parameters causing canine thromboelastographic hypercoagulability and to investigate whether thromboelastography (TEG) with Cytochalasin D (Cyt D) added is related to parameters of platelet activity. Design: Prospective observational study on hemostatic and inflammatory parameters. Data were collected between November 2012 and July 2013. Setting: University teaching hospital. Animals: Twenty-eight dogs suffering from diseases predisposing to thrombosis and 19 clinically healthy dogs. Diseased dogs were enrolled if they fulfilled inclusion criteria regarding age, size, informed client consent, and obtained a diagnosis of a disease that has been associated with thrombosis or hypercoagulability. Interventions: None. Measurements and Main Results: Parameters of coagulation and anticoagulation, fibrinolysis, and antifibrinolysis, platelet activity, inflammation, platelet count, and hematocrit were measured using CBC, TEG, platelet aggregation on multiplate, platelet activity on flow cytometry, and hemostatic and inflammatory markers on plasma and serum analyses. ANOVA and multilinear regression analyses indicated that especially hematocrit and the inflammatory parameters C-reactive protein and interleukin-8 showed best association with overall clot strength in diseased dogs with hypercoagulable TEG tracings. Ratios presumed to reflect platelet contribution to the TEG tracing obtained in TEG analyses with Cyt D were related especially with hematocrit and P-selectin expression of platelets measured after γ-Thrombin activation on flow cytometry. Conclusion: Overall clot strength in TEG analyses of the hypercoagulable dogs included in the present study appears to be primarily associated with inflammation as well as hematocrit. Furthermore, the ratio between standard TEG analyses and TEG analyses with Cyt D may reflect some degree of platelet activity.

AB - Objective: To investigate parameters causing canine thromboelastographic hypercoagulability and to investigate whether thromboelastography (TEG) with Cytochalasin D (Cyt D) added is related to parameters of platelet activity. Design: Prospective observational study on hemostatic and inflammatory parameters. Data were collected between November 2012 and July 2013. Setting: University teaching hospital. Animals: Twenty-eight dogs suffering from diseases predisposing to thrombosis and 19 clinically healthy dogs. Diseased dogs were enrolled if they fulfilled inclusion criteria regarding age, size, informed client consent, and obtained a diagnosis of a disease that has been associated with thrombosis or hypercoagulability. Interventions: None. Measurements and Main Results: Parameters of coagulation and anticoagulation, fibrinolysis, and antifibrinolysis, platelet activity, inflammation, platelet count, and hematocrit were measured using CBC, TEG, platelet aggregation on multiplate, platelet activity on flow cytometry, and hemostatic and inflammatory markers on plasma and serum analyses. ANOVA and multilinear regression analyses indicated that especially hematocrit and the inflammatory parameters C-reactive protein and interleukin-8 showed best association with overall clot strength in diseased dogs with hypercoagulable TEG tracings. Ratios presumed to reflect platelet contribution to the TEG tracing obtained in TEG analyses with Cyt D were related especially with hematocrit and P-selectin expression of platelets measured after γ-Thrombin activation on flow cytometry. Conclusion: Overall clot strength in TEG analyses of the hypercoagulable dogs included in the present study appears to be primarily associated with inflammation as well as hematocrit. Furthermore, the ratio between standard TEG analyses and TEG analyses with Cyt D may reflect some degree of platelet activity.

KW - Cytochalasin D

KW - dog

KW - platelet activity

KW - TEG

KW - thromboelastography

KW - thrombosis

U2 - 10.1111/vec.12675

DO - 10.1111/vec.12675

M3 - Journal article

C2 - 29171709

AN - SCOPUS:85040119971

SN - 1476-4431

VL - 28

SP - 20

EP - 30

JO - Journal of Veterinary Emergency and Critical Care (Online Edition)

JF - Journal of Veterinary Emergency and Critical Care (Online Edition)

IS - 1

ER -

The influence of inflammation and hematocrit on clot strength in canine thromboelastographic hypercoagulability

Abstract

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