The immunoglobulin superfamily member CD200R identifies cells involved in type 2 immune responses

TY - JOUR

T1 - The immunoglobulin superfamily member CD200R identifies cells involved in type 2 immune responses

AU - Blom, Lars H

AU - Martel, Britta C

AU - Larsen, Lau F

AU - Hansen, Camilla V

AU - Christensen, Malene P

AU - Juel-Berg, Nanna

AU - Litman, Thomas

AU - Poulsen, Lars K

N1 - This article is protected by copyright. All rights reserved.

PY - 2017/7

Y1 - 2017/7

N2 - Background: The pathology of allergic diseases involves type 2 immune cells, such as Th2, ILC2, and basophils exerting their effect by production of IL-4, IL-5, and IL-13. However, surface receptors that are specifically expressed on type 2 immune cells are less well documented. The aim of this investigation was to identify surface markers associated with type 2 inflammation. Methods: Naïve human CD4+ T cells were short-term activated in the presence or absence of IL-4 and analyzed for expression of >300 cell-surface proteins. Ex vivo-isolated peripheral blood mononuclear cells (PBMCs) from peanut-allergic (PA) and nonallergic subjects were stimulated (14–16 h) with peanut extract to detect peanut-specific CD4+CD154+ T cells. Biopsies were obtained for transcriptomic analysis from healthy controls and patients with extrinsic or intrinsic atopic dermatitis (AD) and psoriasis. Results: Expression analysis of >300 surface proteins enabled identification of IL-4-upregulated surface proteins, such as CD90, CD108, CD109, and CD200R (CD200R1). Additional analysis of in vitro-differentiated Th0, Th1, and Th2 cultures identified CD200R as upregulated on Th2 cells. From ex vivo-isolated PBMCs, we found high expression of CD200R on Th2 and ILC2 cells and basophils. In PA subjects, the peanut-specific Th2 (CD154+CRTh2+) cells expressed more CD200R than the non-allergen-specific Th2 (CD154−CRTh2+) cells. Moreover, costaining of CD161 and CD200R identified peanut-specific highly differentiated IL-4+IL-5+ Th2 cells. Finally, transcriptomic analysis revealed upregulation of CD200R in lesional skin from subjects with an extrinsic AD phenotype compared to healthy skin. Conclusion: These results indicate that CD200R expression strongly correlates with Th2 pathology; though, the mechanism is as yet elusive.

AB - Background: The pathology of allergic diseases involves type 2 immune cells, such as Th2, ILC2, and basophils exerting their effect by production of IL-4, IL-5, and IL-13. However, surface receptors that are specifically expressed on type 2 immune cells are less well documented. The aim of this investigation was to identify surface markers associated with type 2 inflammation. Methods: Naïve human CD4+ T cells were short-term activated in the presence or absence of IL-4 and analyzed for expression of >300 cell-surface proteins. Ex vivo-isolated peripheral blood mononuclear cells (PBMCs) from peanut-allergic (PA) and nonallergic subjects were stimulated (14–16 h) with peanut extract to detect peanut-specific CD4+CD154+ T cells. Biopsies were obtained for transcriptomic analysis from healthy controls and patients with extrinsic or intrinsic atopic dermatitis (AD) and psoriasis. Results: Expression analysis of >300 surface proteins enabled identification of IL-4-upregulated surface proteins, such as CD90, CD108, CD109, and CD200R (CD200R1). Additional analysis of in vitro-differentiated Th0, Th1, and Th2 cultures identified CD200R as upregulated on Th2 cells. From ex vivo-isolated PBMCs, we found high expression of CD200R on Th2 and ILC2 cells and basophils. In PA subjects, the peanut-specific Th2 (CD154+CRTh2+) cells expressed more CD200R than the non-allergen-specific Th2 (CD154−CRTh2+) cells. Moreover, costaining of CD161 and CD200R identified peanut-specific highly differentiated IL-4+IL-5+ Th2 cells. Finally, transcriptomic analysis revealed upregulation of CD200R in lesional skin from subjects with an extrinsic AD phenotype compared to healthy skin. Conclusion: These results indicate that CD200R expression strongly correlates with Th2 pathology; though, the mechanism is as yet elusive.

U2 - 10.1111/all.13129

DO - 10.1111/all.13129

M3 - Journal article

C2 - 28106273

SN - 0105-4538

VL - 72

SP - 1081

EP - 1090

JO - Allergy

JF - Allergy

IS - 7

ER -