The galactophilic lectin (PA-IL, gene LecA) from Pseudomonas aeruginosa. Its binding requirements and the localization of lectin receptors in various mouse tissues

TY - JOUR

T1 - The galactophilic lectin (PA-IL, gene LecA) from Pseudomonas aeruginosa. Its binding requirements and the localization of lectin receptors in various mouse tissues

AU - Kirkeby, Svend

AU - Hansen, Axel K

AU - d'Apice, Anthony

AU - Moe, Dennis

N1 - Keywords: Adhesins, Bacterial; Adrenal Glands; Animals; Capillaries; Carbohydrate Sequence; Glycoproteins; Heart; Histocytochemistry; Inhibitory Concentration 50; Kidney; Lectins; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Pseudomonas aeruginosa; Receptors, Mitogen

PY - 2006

Y1 - 2006

N2 - The opportunistic pathogen Pseudomonas aeruginosa contains lectins of which one of them, PA-IL (gene lecA), shows preference for alpha-galactosylated glycans. The bacterial lectin is probably important in the carbohydrate-mediated adhesion of the microorganism to endothelia and epithelia and thereby the lectin facilitates entering and damaging of the cells. The requirements for the interaction between PA-IL and the carbohydrate epitopes to which the bacterial lectin may bind were here Studied using alpha-galactosylated neoglycoproteins that were immobilized on Microtiter plates. It is concluded that the carbohydrate recognizing site of the lectin can have a binding requirement of only one saccharide. Lectin histochemistry was performed on sections from wild type mice and from knock-out mice, which lack function of the alpha.1,3-galactosyltransferase gene. All assays with the P. aeruginosa lectin were compared with the results obtained using an isolectin from the legume shrub Griffonia simplicifolia: the GSI-134 isolectin, which is highly specific for glycans terminating in Ga1 alpha 1-R. In the wild-type mice, lectin histochemistry showed a strong capillary reaction in heart, kidney and adrenal gland while none of the two lectins were able to detect capillaries in the pancreas. This could indicate a differential glycosylation with respect to endothelial cell Gal alpha. epitopes among different organs. Further, since no PA-IL bindingto the endothelial cells in the KO mouse was observed, it seems that, in the mouse, the Pseudomonas lectin adheres to the Gal alpha l-3Ga4 beta 1-G1cNAc carbohydrate on endothelial cells in most organs and tissues. Finally, lectin staining of the basement membrane of the acini in the exocrine pancreas suggests the presence of Ga1 alpha 1-3Gal epitopes in WT mice basement membranes that are not detected by the P.aeruginoso lectin.

AB - The opportunistic pathogen Pseudomonas aeruginosa contains lectins of which one of them, PA-IL (gene lecA), shows preference for alpha-galactosylated glycans. The bacterial lectin is probably important in the carbohydrate-mediated adhesion of the microorganism to endothelia and epithelia and thereby the lectin facilitates entering and damaging of the cells. The requirements for the interaction between PA-IL and the carbohydrate epitopes to which the bacterial lectin may bind were here Studied using alpha-galactosylated neoglycoproteins that were immobilized on Microtiter plates. It is concluded that the carbohydrate recognizing site of the lectin can have a binding requirement of only one saccharide. Lectin histochemistry was performed on sections from wild type mice and from knock-out mice, which lack function of the alpha.1,3-galactosyltransferase gene. All assays with the P. aeruginosa lectin were compared with the results obtained using an isolectin from the legume shrub Griffonia simplicifolia: the GSI-134 isolectin, which is highly specific for glycans terminating in Ga1 alpha 1-R. In the wild-type mice, lectin histochemistry showed a strong capillary reaction in heart, kidney and adrenal gland while none of the two lectins were able to detect capillaries in the pancreas. This could indicate a differential glycosylation with respect to endothelial cell Gal alpha. epitopes among different organs. Further, since no PA-IL bindingto the endothelial cells in the KO mouse was observed, it seems that, in the mouse, the Pseudomonas lectin adheres to the Gal alpha l-3Ga4 beta 1-G1cNAc carbohydrate on endothelial cells in most organs and tissues. Finally, lectin staining of the basement membrane of the acini in the exocrine pancreas suggests the presence of Ga1 alpha 1-3Gal epitopes in WT mice basement membranes that are not detected by the P.aeruginoso lectin.

KW - Former LIFE faculty

KW - Lectin

KW - Mouse

KW - alpha-gal epitope

KW - Pseudomonas aeruginosa

U2 - 10.1016/j.micpath.2006.01.006

DO - 10.1016/j.micpath.2006.01.006

M3 - Journal article

C2 - 16542817

SN - 0882-4010

VL - 40

SP - 191

EP - 197

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

IS - 5

ER -