The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently

TY - JOUR

T1 - The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently

AU - Chen, Yongshuo

AU - Li, Shizhong

AU - Berezin, Vladimir

AU - Bock, Elisabeth

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Activation of fibroblast growth factor (FGF) receptors (FGFRs) both by FGFs and by the neural cell adhesion molecule (NCAM) is crucial in the development and function of the nervous system. We found that FGFR substrate 2a (FRS2α), Src homologous and collagen A (ShcA), and phospholipase-Cγ (PLCγ) were all required for neurite outgrowth from cerebellar granule neurons (CGNs) induced by FGF1 and FGL (an NCAM-derived peptide agonist of FGFR1). Like FGF1, FGL induced tyrosine phosphorylation of FGFR1, FRS2α, ShcA, and PLCγ in a time- and dose-dependent manner. However, the activation of FRS2α by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor.

AB - Activation of fibroblast growth factor (FGF) receptors (FGFRs) both by FGFs and by the neural cell adhesion molecule (NCAM) is crucial in the development and function of the nervous system. We found that FGFR substrate 2a (FRS2α), Src homologous and collagen A (ShcA), and phospholipase-Cγ (PLCγ) were all required for neurite outgrowth from cerebellar granule neurons (CGNs) induced by FGF1 and FGL (an NCAM-derived peptide agonist of FGFR1). Like FGF1, FGL induced tyrosine phosphorylation of FGFR1, FRS2α, ShcA, and PLCγ in a time- and dose-dependent manner. However, the activation of FRS2α by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor.

KW - Adaptor Proteins, Signal Transducing

KW - Animals

KW - Cell Enlargement

KW - Cells, Cultured

KW - Cerebellum

KW - Fibroblast Growth Factor 1

KW - Models, Neurological

KW - Neural Cell Adhesion Molecules

KW - Neurites

KW - Neurons

KW - Phospholipase C gamma

KW - Phosphorylation

KW - Rats

KW - Rats, Wistar

KW - Receptors, Fibroblast Growth Factor

KW - Shc Signaling Adaptor Proteins

KW - Time Factors

KW - Tyrosine

U2 - 10.1002/jnr.22374

DO - 10.1002/jnr.22374

M3 - Journal article

C2 - 20175207

SN - 0360-4012

VL - 88

SP - 1882

EP - 1889

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

IS - 9

ER -