TY - JOUR
T1 - The evidence for increased L1 activity in the site of human adult brain neurogenesis
AU - Kurnosov, Alexey A
AU - Ustyugova, Svetlana V
AU - Nazarov, Vadim I
AU - Minervina, Anastasia A
AU - Komkov, Alexander Yu
AU - Shugay, Mikhail
AU - Pogorelyy, Mikhail V
AU - Khodosevich, Konstantin V
AU - Mamedov, Ilgar Z
AU - Lebedev, Yuri B
PY - 2015/2/17
Y1 - 2015/2/17
N2 - Retroelement activity is a common source of polymorphisms in human genome. The mechanism whereby retroelements contribute to the intraindividual genetic heterogeneity by inserting into the DNA of somatic cells is gaining increasing attention. Brain tissues are suspected to accumulate genetic heterogeneity as a result of the retroelements somatic activity. This study aims to expand our understanding of the role retroelements play in generating somatic mosaicism of neural tissues. Whole-genome Alu and L1 profiling of genomic DNA extracted from the cerebellum, frontal cortex, subventricular zone, dentate gyrus, and the myocardium revealed hundreds of somatic insertions in each of the analyzed tissues. Interestingly, the highest concentration of such insertions was detected in the dentate gyrus-the hotspot of adult neurogenesis. Insertions of retroelements and their activity could produce genetically diverse neuronal subsets, which can be involved in hippocampal-dependent learning and memory.
AB - Retroelement activity is a common source of polymorphisms in human genome. The mechanism whereby retroelements contribute to the intraindividual genetic heterogeneity by inserting into the DNA of somatic cells is gaining increasing attention. Brain tissues are suspected to accumulate genetic heterogeneity as a result of the retroelements somatic activity. This study aims to expand our understanding of the role retroelements play in generating somatic mosaicism of neural tissues. Whole-genome Alu and L1 profiling of genomic DNA extracted from the cerebellum, frontal cortex, subventricular zone, dentate gyrus, and the myocardium revealed hundreds of somatic insertions in each of the analyzed tissues. Interestingly, the highest concentration of such insertions was detected in the dentate gyrus-the hotspot of adult neurogenesis. Insertions of retroelements and their activity could produce genetically diverse neuronal subsets, which can be involved in hippocampal-dependent learning and memory.
KW - Brain/physiology
KW - Dentate Gyrus/physiology
KW - Genome, Human
KW - Humans
KW - Long Interspersed Nucleotide Elements/physiology
KW - Neurogenesis/genetics
KW - Neurons/physiology
KW - Promoter Regions, Genetic
U2 - 10.1371/journal.pone.0117854
DO - 10.1371/journal.pone.0117854
M3 - Journal article
C2 - 25689626
SN - 1932-6203
VL - 10
SP - e0117854
JO - PLoS Computational Biology
JF - PLoS Computational Biology
IS - 2
ER -