The effect of insulin resistance and exercise on the percentage of CD16(+) monocyte subset in obese individuals

TY - JOUR

T1 - The effect of insulin resistance and exercise on the percentage of CD16(+) monocyte subset in obese individuals

AU - de Matos, Mariana A

AU - Duarte, Tamiris C

AU - Ottone, Vinícius de O

AU - Sampaio, Pâmela F da M

AU - Costa, Karine B

AU - de Oliveira, Marcos F Andrade

AU - Moseley, Pope L

AU - Schneider, Suzanne M

AU - Coimbra, Cândido C

AU - Brito-Melo, Gustavo E A

AU - Magalhães, Flávio de C

AU - Amorim, Fabiano T

AU - Rocha-Vieira, Etel

N1 - Copyright © 2016 John Wiley & Sons, Ltd.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Obesity is a low-grade chronic inflammation condition, and macrophages, and possibly monocytes, are involved in the pathological outcomes of obesity. Physical exercise is a low-cost strategy to prevent and treat obesity, probably because of its anti-inflammatory action. We evaluated the percentage of CD16− and CD16+ monocyte subsets in obese insulin-resistant individuals and the effect of an exercise bout on the percentage of these cells. Twenty-seven volunteers were divided into three experimental groups: lean insulin sensitive, obese insulin sensitive and obese insulin resistant. Venous blood samples collected before and 1 h after an aerobic exercise session on a cycle ergometer were used for determination of monocyte subsets by flow cytometry. Insulin-resistant obese individuals have a higher percentage of CD16+ monocytes (14.8 ± 2.4%) than the lean group (10.0 ± 1.3%). A positive correlation of the percentage of CD16+ monocytes with body mass index and fasting plasma insulin levels was found. One bout of moderate exercise reduced the percentage of CD16+ monocytes by 10% in all the groups evaluated. Also, the absolute monocyte count, as well as all other leukocyte populations, in lean and obese individuals, increased after exercise. This fact may partially account for the observed reduction in the percentage of CD16+ cells in response to exercise. Insulin-resistant, but not insulin-sensitive obese individuals, have an increased percentage of CD16+ monocytes that can be slightly modulated by a single bout of moderate aerobic exercise. These findings may be clinically relevant to the population studied, considering the involvement of CD16+ monocytes in the pathophysiology of obesity.

AB - Obesity is a low-grade chronic inflammation condition, and macrophages, and possibly monocytes, are involved in the pathological outcomes of obesity. Physical exercise is a low-cost strategy to prevent and treat obesity, probably because of its anti-inflammatory action. We evaluated the percentage of CD16− and CD16+ monocyte subsets in obese insulin-resistant individuals and the effect of an exercise bout on the percentage of these cells. Twenty-seven volunteers were divided into three experimental groups: lean insulin sensitive, obese insulin sensitive and obese insulin resistant. Venous blood samples collected before and 1 h after an aerobic exercise session on a cycle ergometer were used for determination of monocyte subsets by flow cytometry. Insulin-resistant obese individuals have a higher percentage of CD16+ monocytes (14.8 ± 2.4%) than the lean group (10.0 ± 1.3%). A positive correlation of the percentage of CD16+ monocytes with body mass index and fasting plasma insulin levels was found. One bout of moderate exercise reduced the percentage of CD16+ monocytes by 10% in all the groups evaluated. Also, the absolute monocyte count, as well as all other leukocyte populations, in lean and obese individuals, increased after exercise. This fact may partially account for the observed reduction in the percentage of CD16+ cells in response to exercise. Insulin-resistant, but not insulin-sensitive obese individuals, have an increased percentage of CD16+ monocytes that can be slightly modulated by a single bout of moderate aerobic exercise. These findings may be clinically relevant to the population studied, considering the involvement of CD16+ monocytes in the pathophysiology of obesity.

KW - Adolescent

KW - Adult

KW - Blood Cell Count

KW - Demography

KW - Exercise

KW - Female

KW - Humans

KW - Insulin Resistance

KW - Male

KW - Middle Aged

KW - Monocytes/pathology

KW - Obesity/pathology

KW - Receptors, IgG/metabolism

KW - Young Adult

U2 - 10.1002/cbf.3178

DO - 10.1002/cbf.3178

M3 - Journal article

C2 - 27027694

SN - 0263-6484

VL - 34

SP - 209

EP - 216

JO - Cell Biochemistry and Function

JF - Cell Biochemistry and Function

IS - 4

ER -