The effect of different blood sampling sites and analyses on the relationship~ between exercise intensity and 4.0 mmol ·1^- blood lactate concentration

TY - JOUR

T1 - The effect of different blood sampling sites and analyses on the relationship~ between exercise intensity and 4.0 mmol ·1- blood lactate concentration

AU - Foxdal, P

AU - Sjödin, Anders Mikael

AU - Östman, B

AU - Sjödin, B

PY - 1991

Y1 - 1991

N2 - The aim of the study was to examine whether the difference in lactate concentration in different blood fractions is of practical importance when using blood lactate as a test variable of aerobic endurance capacity. Ten male firefighters performed submaximally graded exercise on a cycle ergometer for 20-25 min. Venous and capillary blood samples were taken every 5 min for determination of haematocrit and lactate concentrations in plasma, venous and capillary blood. At the same time, expired air was collected in Douglas bags for determination of the oxygen consumption. A lactate concentration of 4.0 mmol·1-1 was used as the reference value toi compare the oxygen consumption and exercise intensity when different types of blood specimen and sampling sites were used for lactate analysis. At this concentration the exercise intensity was 17% lower (P<0.01) when plasma lactate was compared toi venous blood lactate, and 12% lower (P<0.05) when capillary blood lactate was used. Similar discrepancies were seen in oxygen consumption. The results illustrated the importance of standardizing sampling and handling of blood specimens for lactate determination to enable direct comparisons to be made among results obtained in different studies.

AB - The aim of the study was to examine whether the difference in lactate concentration in different blood fractions is of practical importance when using blood lactate as a test variable of aerobic endurance capacity. Ten male firefighters performed submaximally graded exercise on a cycle ergometer for 20-25 min. Venous and capillary blood samples were taken every 5 min for determination of haematocrit and lactate concentrations in plasma, venous and capillary blood. At the same time, expired air was collected in Douglas bags for determination of the oxygen consumption. A lactate concentration of 4.0 mmol·1-1 was used as the reference value toi compare the oxygen consumption and exercise intensity when different types of blood specimen and sampling sites were used for lactate analysis. At this concentration the exercise intensity was 17% lower (P<0.01) when plasma lactate was compared toi venous blood lactate, and 12% lower (P<0.05) when capillary blood lactate was used. Similar discrepancies were seen in oxygen consumption. The results illustrated the importance of standardizing sampling and handling of blood specimens for lactate determination to enable direct comparisons to be made among results obtained in different studies.

KW - Blood lactate

KW - Blood sampling sites

KW - Exercise intensity

U2 - 10.1007/BF00760801

DO - 10.1007/BF00760801

M3 - Journal article

C2 - 1915332

AN - SCOPUS:0025784386

SN - 0301-5548

VL - 63

SP - 52

EP - 54

JO - European Journal of Applied Physiology and Occupational Physiology

JF - European Journal of Applied Physiology and Occupational Physiology

IS - 1

ER -